Results 161 to 170 of about 206,672 (309)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Tests for Categorical Data Beyond Pearson: A Distance Covariance and Energy Distance Approach. [PDF]

Biom J
Castro-Prado F, González-Manteiga W, Costas J, Facal F, Edelmann D.   +4 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Structure-preserving multivariate hypothesis testing for mass spectrometry imaging and single-cell data. [PDF]

Bioinformatics
Liebenberg KE, Craig E, Tibshirani R, Eberlin LS.   +3 more
europepmc   +1 more source

Min-wise independent permutations (extended abstract)

Symposium on the Theory of Computing, 1998
A. Broder, M. Charikar, A. Frieze, M. Mitzenmacher   +3 more
semanticscholar   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Brain structural morphometry in non-musicians with superior pitch identification ability. [PDF]

Sci Rep
Hou J, Hosseini T, O'Boyle MW.
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Concordance between nutritional cohorts and randomized trials: biological confirmation or statistical consequence? A re-analysis of Stadelmaier et al. [PDF]

BMC Med
Calkins M, Núñez I, Soto-Mota A.
europepmc   +1 more source

Constructing new differentially 4-uniform permutations from the inverse function

Finite Fields Their Appl., 2014
Zhengbang Zha, Lei Hu, Siwei Sun
semanticscholar   +1 more source