Results 41 to 50 of about 87,496 (253)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Field Evaluation of Grapevine Rootstocks Inoculated with Fungi Associated with Petri Disease and Esca

American Journal of Enology and Viticulture, 2010
One-year-old grapevine rootstock cuttings (41 B Millardet-Grasset, 140 Ruggeri, 161 49 Couderc, 1103 Paulsen and 110 Richter) were inoculated with pathogens associated with Petri disease and esca of grapevine, to determine the effects of fungal infection on percentage of cuttings emerging from dormancy, shoot weight and disease severity.
Gramaje, David, García-Jiménez, José, Armengol Fortí, Josep   +2 more
openaire   +2 more sources

Hydrostatic pressure activates HIF‐1α via β‐catenin to promote stemness in breast cancer cells

FEBS Open Bio, EarlyView.
To mimic the elevated intestinal fluid pressure in breast cancers, we loaded human breast cancer cells (MCF‐7, MDA‐MB‐453, and BT‐474) to 50 mmHg hydrostatic pressure. Hydrostatic pressure exposure upregulated HIF‐1α and induced stemness in MCF‐7 and BT‐474 cells.
Da Zhai, Yong Xu, Jingjing Ma, Ratchanee Duangrat, Chen Yan, Jingyan Liang, Hua Wei, Bin Jiang, Tao‐Sheng Li   +8 more
wiley   +1 more source

Root rot and damping-off of Aleppo pine seedlings caused by Pythium spp. in Algerian forest nurseries

Journal of Forest Science, 2016
Aleppo pine (Pinus halepensis Miller) is a common forest tree in the Mediterranean Region. Production of Aleppo pine seedlings is hindered by damping off and root rot diseases in cooler conditions of north-western Algeria, which significantly reduces the
F. Lazreg, L. Belabid, J. Sánchez, E. Gallego   +3 more
doaj   +1 more source

Risk of Retinopathy Associated with Long‐Term Use of Hydroxychloroquine in Patients with Rheumatic Diseases: A Systematic Review and Meta‐Analysis

Arthritis Care &Research, EarlyView.
Objective We aimed to estimate the prevalence and cumulative incidence of hydroxychloroquine retinopathy (HCQ‐R) and its risk factors among patients receiving long‐term HCQ with rheumatic diseases through a systematic review and meta‐analysis of observational studies that used spectral‐domain optical coherence tomography (SD‐OCT) for screening ...
Narsis Daftarian, Chloe Yue, Steve D Levasseur, Hui Xie, J. Antonio Avina‐Zubieta   +4 more
wiley   +1 more source

Clinical, Histologic, and Serological Predictors of Renal Function Loss in Lupus Nephritis

Arthritis Care &Research, EarlyView.
Objective Kidney survival is the ultimate goal in lupus nephritis (LN) management, but long‐term predictors remain inadequately studied, requiring long‐term follow‐up. This study aimed to identify baseline and early longitudinal predictors of kidney survival in the Accelerating Medicines Partnership LN longitudinal cohort.
Shangzhu Zhang, Laurence Magder, Daniel Goldman, Judith A. James, Joel M. Guthridge, Carla Guthridge, Peter Izmirly, Jill P. Buyon, H. Michael Belmont, Richard A. Furie, Noa Schwartz, Chaim Putterman, Jennifer L. Barnas, Jennifer H. Anolik, Sarah French, Maria Dall'Era, Avi Z. Rosenberg, Jeffrey Hodgin, Dawit S. Demeke, the Accelerating Medicines Partnership Rheumatoid Arthritis and Systemic Lupus Erythematosus (RA/SLE) Network, Michelle Petri, Andrea Fava   +21 more
wiley   +1 more source

Fungi associated with grapevine trunk diseases in nursery-produced Vitis vinifera plants

Phytopathologia Mediterranea, 2018
Grapevine trunk diseases (GTDs) are one of the most important groups of fungal diseases affecting grapevine plants worldwide. One of the main causes of GTDs infection occur during nursery plant production processes.
Cristina PINTOS, Vanesa REDONDO, Daniel COSTAS, Olga AGUÍN, Pedro MANSILLA   +4 more
doaj   +1 more source

Transparent and Robust LiCl–Organohydrogel Triboelectric Nanogenerator With Deep Learning Assisted Sensing

Advanced Functional Materials, EarlyView.
Develop a LiCl–PEI–PAM hydrogel with 3000% stretchability and excellent optical transparency. Through comparative studies of various salts, confirm that LiCl is the most suitable salt for high TENG output. Achieve excellent freeze‐resistant, dry‐resistant, and rapid self‐healing (10 s) properties even in extreme environments. Balance ionic conductivity,
Hai Anh Thi Le, Thien Trung Luu, Duc‐Manh Dinh, Kyehan Rhee, Kyungwho Choi, Dukhyun Choi, Yong Tae Park   +6 more
wiley   +1 more source

Molecular and phenotypic characterisation of Phaeomoniella chlamydospora isolates from the demarcated wine region of Dão (Portugal)

Phytopathologia Mediterranea, 2015
Sixty-eight isolates of Phaeomoniella chlamydospora obtained from symptomatic esca and Petri disease grapevines, obtained mostly within the Portuguese Dão appellation, were investigated regarding their phenotypic and molecular diversity, in order to ...
Jorge SOFIA, João TROVÃO, António PORTUGAL, Hugo PAIVA DE CARVALHO, Nuno MESQUITA, Teresa NASCIMENTO, Cecília REGO, Maria GONÇALVES   +7 more
doaj   +1 more source

Mechanical and Electrical Phenotype of hiPSC‐Cardiomyocytes on Fibronectin‐Based Hydrogels

Advanced Healthcare Materials, EarlyView.
We introduce fibronectin‐based PEG hydrogels with controlled rigidity to enable the culture of iPSC‐derived cardiomyocytes. These substrates offer an alternative to the current culture of these cells on fibronectin‐coated glass, providing enhanced structural and functional behavior. The system provides a more physiologically relevant platform to assess
Ana Da Silva Costa, Lineta Stonkute, Sara Trujillo, Mariana Azevedo Gonzalez Oliva, Francis Burton, Matthew J. Dalby, Oana Dobre, Godfrey Smith, Manuel Salmeron‐Sanchez   +8 more
wiley   +1 more source