Results 121 to 130 of about 488,297 (316)

Pre‐analytical optimization of cell‐free DNA and extracellular vesicle‐derived DNA for mutation detection in liquid biopsies

Molecular Oncology, EarlyView.
Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen, Lucrezia De Santis, Bret M. Stephens, Vincent Bernard, Jörg C. Kalff, Lara Braun, Phillipp Leyendecker, Johannes Röttgen, Daniel Weissinger, Anirban Maitra, Paola A. Guerrero, Alexander Semaan   +11 more
wiley   +1 more source

Congenital External Auditory Canal Atresia in Two Dogs: Clinical Presentation, Diagnosis, and Surgical Management

Pets
Congenital external auditory canal atresia (EACA) is a rare developmental anomaly in dogs with limited information to guide management. This report describes the clinical presentation, diagnostic work-up, surgical technique, and long-term outcome in two ...
Mandalena Markou, Thomas Koutis, Konstantina Karagianni, Ioannis Panopoulos, Eugenia Flouraki, Vassiliki Tsioli   +5 more
doaj   +1 more source

Deciphering transcriptional plasticity in pancreatic ductal adenocarcinoma reveals alterations in sensory neuron innervation

Molecular Oncology, EarlyView.
Pancreatic sensory neurons innervating healthy and PDAC tissue were retrogradely labeled and profiled by single‐cell RNA sequencing. Tumor‐associated innervation showed a dominant neurofilament‐positive subtype, altered mitochondrial gene signatures, and reduced non‐peptidergic neurons.
Elena Genova, Michele Montrone, Uday Rangaswamy, Francesco Diversi, Irene Schiavo, Denise Ferrarini, Roberta Di Florio, Irene Longo, Michele Coscia, Nicola Zamboni, Giorgia Demontis, Lisa Veghini, Vincenzo Corbo, Remo Sanges, Paul Heppenstall   +14 more
wiley   +1 more source

Effect of a Supplement Containing Probiotics, Prebiotics, and Yeast Extract on Gut Inflammation, Microbiota, and Cytokines in Healthy Dogs

Pets
Probiotics, prebiotics, and postbiotics are of interest for their potential gastrointestinal and immunological benefits in pet health. This study aimed to assess whether a unique blend of Bacillus subtilis, Bacillus clausii, Bacillus coagulans ...
Angela Zilinger, Mary K. Sramek, Tarun Chandra, Teresa Schmidt, Jessica Bagel, Andrew Stayduhar, James Fryer, Gregory D. Sunvold   +7 more
doaj   +1 more source

E2A selectively regulates TGF‐β–induced apoptosis in KRAS‐mutant non‐small cell lung cancer

Molecular Oncology, EarlyView.
Ability to induce apoptosis by TGF‐β is frequently lost in advanced lung adenocarcinoma despite intact TGF‐β signaling. We identify E2A as a mutant KRAS–dependent mediator of resistance to TGF‐β–induced apoptosis. TGF‐β induces E2A via SMAD3 in mutant KRAS cells, and E2A silencing restores apoptosis and enhances radiation response in cell lines ...
Sergei Chuikov, Shiva Krishna Katkam, Zhefan Wang, Venkateshwar G. Keshamouni   +3 more
wiley   +1 more source

Characterization of Clinical, Hematological, and Biochemical Findings in Dogs with Vipera aspis Envenomation

Pets
Viper envenomation in dogs represents a significant medical emergency in regions where vipers are endemic. Despite its clinical relevance, detailed data on the haematological and biochemical alterations in canine viper envenomation remain limited.
Filomena Meduri, Claudia Rifici, Pietro Gambadauro, Diego Antonio Sicuso, Gianluca Novari, Giuseppe Mazzullo, Michela Pugliese   +6 more
doaj   +1 more source

Rethinking plastic waste: innovations in enzymatic breakdown of oil‐based polyesters and bioplastics

FEBS Open Bio, EarlyView.
Plastic pollution remains a critical environmental challenge, and current mechanical and chemical recycling methods are insufficient to achieve a fully circular economy. This review highlights recent breakthroughs in the enzymatic depolymerization of both oil‐derived polyesters and bioplastics, including high‐throughput protein engineering, de novo ...
Elena Rosini, Nicolò Antonelli, Gianluca Molla   +2 more
wiley   +1 more source

Adverse Reactions to Yeast Beta-Glucan Supplementation in Two Dogs with Inflammatory Bowel Disease

Pets
Beta-glucans are immunomodulatory compounds known to act as adjuvants in the beneficial regulation of various allergic conditions, particularly atopic dermatitis, as well as in the modulation of dysbiosis in patients with chronic gastrointestinal ...
Andressa Rodrigues Amaral, Pedro Henrique Marchi, Natália Manuela Cardoso de Oliveira, Beatriz Leme do Carmo, Larissa Wünsche Risolia, Carlos Eduardo Larsson, Júlio Cesar de Carvalho Balieiro, Thiago Henrique Annibale Vendramini   +7 more
doaj   +1 more source

Overview of molecular signatures of senescence and associated resources: pros and cons

FEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas, Sylvia Vagena, Pavlos Pantelis, Giorgos Theocharous, Russel Petty, Konstantinos Evangelou, Vassilis G. Gorgoulis   +6 more
wiley   +1 more source

Knowledge, Attitudes, and Practices of Pet Owners Regarding Pet Insurance in Selangor, Malaysia

Pets
Pet insurance is a financial tool meant to provide financial support to pet owners for veterinary expenses. It was introduced to the Malaysian market in 2010 with several companies offering various subscription packages.
Audrey Koh Rui Ping, Shamsaldeen Ibrahim Saeed, Mohammed D. Goni, Nor Fadhilah Kamaruzzaman   +3 more
doaj   +1 more source