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Production of PfEMP1-Specific Mouse Monoclonal Antibodies
2022The PfEMP1 family of proteins expressed on the Plasmodium falciparum-infected erythrocyte (IE) surface is the main target of naturally acquired immunity against malaria. Antibodies capable of opsonizing the IEs and blocking the binding between PfEMP1 and human receptors seems to be one of the main protective mechanisms of the naturally acquired ...
Dalgaard, Nanna, Barfod, Lea
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Host receptor binding induces conformational changes in a PfEMP1 protein
Structure, 2023In this issue of Structure, Raghavan et al. present the cryo-EM structures of the malaria-associated group A PfEMP1 HB3VAR03 head in both host receptor-free and receptor-bound states. The structures are complemented by biophysical analysis and introduce an innovative model in which host-receptor binding induces conformational changes in a PfEMP1 ...
Rui, Ma, Niraj H, Tolia
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Surface Plasmon Resonance Analysis of PfEMP1 Interaction with Receptors
2022A detailed understanding of the interaction between the highly variant Plasmodium falciparum erythrocyte membrane proteins 1 (PfEMP1) and their human binding partners is essential to explain their roles in disease development in malaria, as well as to understand how antibodies can inhibit these interactions and how the parasite manages to evade such an
Frank, Lennartz, Matthew K, Higgins
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Affinity Purification of PfEMP1-Specific Antibodies from Human Blood
2022Acquired immunity against Plasmodium falciparum infections relies heavily on IgG antibodies specific for PfEMP1 proteins expressed on the surface of infected erythrocytes. Purified human antibodies can be used, for example, to study the interactions between specific PfEMP1 proteins and receptors expressed by human endothelial cells, and to identify ...
Rebecca W. Olsen +2 more
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var genes, PfEMP1 and the human host
Molecular and Biochemical Parasitology, 2004Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is an important virulence factor encoded by a family of roughly 60 var genes and is used by the parasite to interact with the human host. The parasite regularly exchanges the expressed var gene generating antigenic variation of the infected RBCs (pRBC) surface which is crucial for successful
Kirsten, Flick, Qijun, Chen
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Production of anti-PfEMP1 Polyclonal Antisera in Rats and Mice
2022Plasmodium falciparum-infected erythrocytes (IEs) bind various host receptors via members of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family expressed on the surface of the IEs. Antibody reagents are needed to investigate interactions between specific PfEMP1 proteins and receptors expressed by human endothelial cells.
Rebecca W. Olsen +2 more
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Expression of Large Full-Length PfEMP1 Proteins in HEK293 Cells
2022Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is a family of proteins expressed on the surface of red blood cells infected by Plasmodium falciparum. PfEMP1 proteins play a vital role in parasite virulence, and thus are important vaccine candidates to prevent severe disease.
Jonathan Paul Renn +2 more
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Analysis of Antibody Inhibition of PfEMP1 Binding by Competition ELISA
2022Plasmodium falciparum express variant antigens on the surface of infected erythrocytes (IEs). These include proteins of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. PfEMP1 proteins mediate binding of IEs to various human endothelial receptors and induce antibodies response during natural infections.
Rebecca W. Olsen +2 more
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Extraction and Immunoprecipitation of VAR2CSA, the PfEMP1 Associated with Placental Malaria
2022The Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is a key virulence factor for this human malaria parasite. During pregnancy, VAR2CSA is the only PfEMP1 variant expressed on the surface of infected erythrocytes that mediates adhesion to placenta cells and causes severe pregnancy outcomes.In this chapter, we present an optimized ...
Gamain, Benoît +1 more
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Role of Nonimmune IgG Bound to PfEMP1 in Placental Malaria
Science, 2001Infections with Plasmodium falciparum during pregnancy lead to the accumulation of parasitized red blood cells (infected erythrocytes, IEs) in the placenta. IEs of P. falciparum isolates that infect the human placenta were found to bind immunoglobulin G (IgG).
K, Flick +6 more
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