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Production of PfEMP1-Specific Human Monoclonal Antibodies from Naturally Immune Individuals
2022Plasmodium falciparum parasites express variable surface antigens on the infected erythrocyte surface allowing adhesion to human host receptors on the blood and endothelial cells, which can result in immune evasion. One of the most studied and key antigens in adhesion is the highly polymorphic PfEMP1.
Walker, Melanie R, Barfod, Lea
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A family affair: var genes, PfEMP1 binding, and malaria disease
Current Opinion in Microbiology, 2006An immunovariant adhesion protein family in Plasmodium falciparum named erythrocyte membrane protein 1 (PfEMP1), encoded by var genes, is responsible for both antigenic variation and cytoadhesion of infected erythrocytes at blood microvasculature sites throughout the body. Elucidation of the genome sequence of P.
Susan M, Kraemer, Joseph D, Smith
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2022
Plasmodium falciparum expresses a broad range of proteins on the surface of infected erythrocytes (IEs), including members of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. This protocol describes an immunomagnetic selection method using PfEMP1-specific antibodies to obtain a parasite clone homogenously expressing a particular PfEMP1
Mary Lopez-Perez +1 more
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Plasmodium falciparum expresses a broad range of proteins on the surface of infected erythrocytes (IEs), including members of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. This protocol describes an immunomagnetic selection method using PfEMP1-specific antibodies to obtain a parasite clone homogenously expressing a particular PfEMP1
Mary Lopez-Perez +1 more
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Characterization of anti-var2CSA-PfEMP1 cytoadhesion inhibitory mouse monoclonal antibodies
Microbes and Infection, 2006Pregnancy-associated malaria (PAM) is associated with the massive sequestration of erythrocytes infected with CSA-binding parasites in the placenta. Natural protective immunity against PAM is acquired during the course of pregnancies, with the development of anti-PfEMP1 antibodies recognizing placental infected erythrocytes (IEs) from different ...
Marion, Avril +5 more
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Glycophorin B-PfEMP1 interaction mediates robust rosetting in Plasmodium falciparum
International Journal of Biological MacromoleculesP. falciparumerythrocyte membrane protein 1 (PfEMP1) is the major parasite protein responsible for rosetting by binding to host receptors such as heparan sulfate, CR1 on RBC surface. Usually monomeric protein-carbohydrate interactions are weak [1], therefore PfEMP1 binds to plasma proteins like IgM or α2-macroglobulin that facilitate its clustering on ...
Bijayeeta, Deb +6 more
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Expressing Full-Length Functional PfEMP1 Proteins in the HEK293 Expression System
2012Due to the A/T-richness of the genome of Plasmodium falciparum, expressing P. falciparum proteins in heterologous expression systems is challenging. In addition, many P. falciparum proteins have high cysteine content and high molecular weight, which further complicates expression of these proteins in heterologous systems.
Srivastava, Anand +2 more
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2022
Cultures of Plasmodium falciparum often contain a heterogeneous parasite population. However, several studies require analysis of single infected erythrocytes (IEs) or a clonal parasite population derived from a single parasite. This protocol describes an efficient method for cloning by using fluorescence-activated cell sorting (FACS).
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Cultures of Plasmodium falciparum often contain a heterogeneous parasite population. However, several studies require analysis of single infected erythrocytes (IEs) or a clonal parasite population derived from a single parasite. This protocol describes an efficient method for cloning by using fluorescence-activated cell sorting (FACS).
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Molecular mechanisms of PfEMP1-EPCR Adhesion in severe P. falciparum infection
A major global health burden is still posed by P. falciparum malaria, which causes significant morbidity and mortality in cases of severe illness. Recent findings pertaining to the expression of P. falciparum erythrocyte membrane protein 1 (PfEMP1) on infected erythrocytes and their close association with the onset of severe malaria syndromes, along ...Supriya Pal, Savitri Tiwari
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