Results 231 to 240 of about 7,386,473 (331)
Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley +1 more source
A multifaceted molecular approach to surveillance of leishmaniasis: Identification of sand fly species, Leishmania parasites, and blood meal sources using high-resolution melting analysis. [PDF]
Studentsky L+9 more
europepmc +1 more source
Homologous expression and purification of human HAX‐1 for structural studies
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Evidence of cellular adaptations in a fungal cultivar promoting resource exchange with leafcutter ant farmers. [PDF]
Stelate A, Shik JZ.
europepmc +1 more source
Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul+3 more
wiley +1 more source
Effects of biochar on soil properties as well as available and TCLP-extractable Cu contents: a global meta-analysis. [PDF]
Teng X+11 more
europepmc +1 more source