Results 231 to 240 of about 7,386,473 (331)

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

open access: yesFEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Reviewers Vol. 30, no. 1 (2016)

open access: yesPhilologia Hispalensis, 2016
PH PH
doaj  

A multifaceted molecular approach to surveillance of leishmaniasis: Identification of sand fly species, Leishmania parasites, and blood meal sources using high-resolution melting analysis. [PDF]

open access: yesPLoS Negl Trop Dis
Studentsky L   +9 more
europepmc   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

open access: yesFEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Créditos Vol. 14, núm. 1 (2000)

open access: yesPhilologia Hispalensis, 2000
PH PH
doaj  

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

open access: yesFEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul   +3 more
wiley   +1 more source

Cover of Vol. 30, no. 1 (2016)

open access: yesPhilologia Hispalensis, 2016
PH PH
doaj  

Effects of biochar on soil properties as well as available and TCLP-extractable Cu contents: a global meta-analysis. [PDF]

open access: yesSci Rep
Teng X   +11 more
europepmc   +1 more source

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