Results 181 to 190 of about 127,368 (296)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Sol-gel-derived optical oxygen, pH and dissolved carbon dioxide sensors [PDF]

, 2008
Wencel, Dorota
core  

Gelatin Hydrogel pH Sensors Incorporating Anthocyanins for Intelligent Food Packaging: Towards Enhanced Food Spoilage Detection. [PDF]

Gels
Lopes PM, Mare L, Barbu-Tudoran L, Gadja A, Popescu V.   +4 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Capacitive Coulometric Readout of Polyaniline Membrane-Based pH Sensors in Combination with Cyclic Voltammetry and Electrochemical Impedance Spectroscopy. [PDF]

Membranes (Basel)
Han T, Song T, Han D, Niu L.
europepmc   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

7-Amino-2-(<i>N</i>,<i>N</i>-dimethylamino)quinazoline: a small, bright, easily synthesized scaffold for pH sensors. [PDF]

RSC Adv
Mahdaly M, Maki T, Osako R, Fujimaki Y, Suzuki Y.   +4 more
europepmc   +1 more source

Flexible SAW Microfluidic Devices as Wearable pH Sensors Based on ZnO Nanoparticles. [PDF]

Nanomaterials (Basel), 2021
Piro L, Lamanna L, Guido F, Balena A, Mariello M, Rizzi F, De Vittorio M.   +6 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

From Lab to Shelf: Gelatin-Based pH Sensors Revolutionizing Food Packaging. [PDF]

Gels
Wang R.
europepmc   +1 more source