Results 111 to 120 of about 8,870 (219)

Advances in Phaeodactylum tricornutum nuclear engineering

, 2023
The marine diatom Phaeodactylum tricornutum has the potential to become an excellent platform for the sustainable production of valuable compounds and pharmaceuticals, but currently large-scale engineering of this organism remains a challenge due factors like inefficient genetic transformation protocols and a lack of accurate genomic data.
openaire   +1 more source

GG1 - sgRNA cloning for Phaeodactylum tricornutum v1 [PDF]

, 2019
The CRISPR-Cas9 gene mutagenesis system was adapted for the marine diatom Phaeodactylum tricornutum (CCAP-1055/1) (Figure 1). Here, Cas9 and sgRNA(s) were delivered to Phaeodactylum by bacterial-conjugation transformation on an episome, or artificial chromosome, that is stably maintained and replicated independently from and with the Phaeodactylum ...
Mark Moosburner, Andrew E Allen
openaire   +1 more source

Metabolic engineering of omega-3 long chain polyunsaturated fatty acids in plants using different ∆6- and ∆5-desaturases co-expressed with LPCAT from the marine diatom Phaeodactylum tricornutum

Scientific Reports
Continuous research on obtaining an even more efficient production of very long-chain polyunsaturated fatty acids (VLC-PUFAs) in plants remains one of the main challenges of scientists working on plant lipids.
Sylwia Klińska-Bąchor, Kamil Demski, Yangmin Gong, Antoni Banaś   +3 more
doaj   +1 more source

Meta-analysis of RNA-Seq datasets allows a better understanding of P. tricornutum cellular biology, a requirement to improve the production of Biologics

Scientific Reports
The marine diatom Phaeodactylum tricornutum is currently used for various industrial applications, including the pharmaceutical industry as a cost-effective cell biofactory to produce Biologics. Recent studies demonstrated that P. tricornutum can produce
Isabelle Boulogne, Charlotte Toustou, Muriel Bardor   +2 more
doaj   +1 more source

Comparative RNA-Seq of Ten Phaeodactylum tricornutum Accessions: Unravelling Criteria for Robust Strain Selection from a Bioproduction Point of View

Marine Drugs
The production of biologics in mammalian cells is hindered by some limitations including high production costs, prompting the exploration of other alternative expression systems that are cheaper and sustainable like microalgae.
Charlotte Toustou, Isabelle Boulogne, Anne-Alicia Gonzalez, Muriel Bardor   +3 more
doaj   +1 more source

Genome scale metabolic modelling of Phaeodactylum tricornutum

, 2017
Diatoms are photoautotrophic unicellular algae and are among the most abundant, adaptable and diverse marine phytoplankton. Their ability to synthesise lipid as a storage compound (20%-50% dry cell weight) makes them a potential sources of biofuel and high-value commodities such as ω fatty acids.
openaire   +1 more source

Charaterization of the Phaeodactylum tricornutum epigenome

, 2012
La méthylation de l'ADN est l’une des marques épigénétiques les plus étudiées et est largement conservée. Mes travaux de thèse présentent le premier méthylome d'une diatomée marine P. tricornutum qui appartient à la famille des Stramenopiles. P. tricornutum présente une méthylation d’environ 6% qui est présente en mosaïque sur l’ensemble du génome. Une
openaire   +1 more source

Transformation of Phaeodactylum tricornutum by electroporation v1

, 2018
Successful transformation ofPhaeodactylum tricornutum (CMP632) by electroporationwas achieved based on a modified version of Zhang & Hu (2014).
openaire   +1 more source

From isolation to insights: mitochondrial complex I in the diatom Phaeodactylum tricornutum. [PDF]

Plant J
Berdun F, Senkler J, Senkler M, Ditz N, Plönnigs E, Reinard T, Zabaleta E, Braun HP.   +7 more
europepmc   +1 more source

Metabolic engineering for improved heterologous pinene production in the chloroplast of Phaeodactylum tricornutum. [PDF]

Microb Cell Fact
Trevisan N, van der Oost J, Barbosa M, D'Adamo S.   +3 more
europepmc   +1 more source