Results 11 to 20 of about 4,379 (172)

Optimizing CRISPR/Cas9 for the Diatom Phaeodactylum tricornutum

Frontiers in Plant Science, 2018
CRISPR/Cas9 is a powerful tool for genome editing. We constructed an easy-to-handle expression vector for application in the model organism Phaeodactylum tricornutum and tested its capabilities in order to apply CRISPR/Cas9 technology for our purpose. In
Daniel Stukenberg, Stefan Zauner, Gianluca Dell’Aquila, Uwe G. Maier, Uwe G. Maier   +4 more
doaj   +3 more sources

From isolation to insights: mitochondrial complex I in the diatom Phaeodactylum tricornutum. [PDF]

Plant J
SUMMARY Diatoms are among the most ecologically successful microalgae, contributing significantly to marine primary production and global carbon cycling. Their distinctive metabolic architecture, shaped by a complex evolutionary history involving secondary endosymbiosis, includes a highly compartmentalized cell organization and unique metabolic ...
Berdun F, Senkler J, Senkler M, Ditz N, Plönnigs E, Reinard T, Zabaleta E, Braun HP.   +7 more
europepmc   +2 more sources

Transcriptomic Responses of the Marine Diatom <i>Phaeodactylum tricornutum</i> to High Carbon and Low Nitrogen Stress. [PDF]

Ecol Evol
To elucidate the integrated transcriptional mechanisms underlying diatom acclimation to coupled carbon–nitrogen (CN) imbalance, we employed RNA sequencing (RNA‐Seq) to characterize the global transcriptional response of the model diatom Phaeodactylum tricornutum to high CO2 (~2000 μatm) and low nitrogen (10% of nitrogen concentration in f/2 medium ...
Zhang Y, Duan J, Zheng Y, Chen X, Li C, Xie Z, Huang A.   +6 more
europepmc   +2 more sources

Phaeodactylum tricornutum: A Diatom Cell Factory [PDF]

Trends in Biotechnology, 2020
A switch from a petroleum-based to a biobased economy requires the capacity to produce both high-value low-volume and low-value high-volume products. Recent evidence supports the development of microalgae-based microbial cell factories with the objective of establishing environmentally sustainable manufacturing solutions. Diatoms display rich diversity
Butler, T., Kapoore, R.V., Vaidyanathan, S.   +2 more
openaire   +4 more sources

Observations on Phaeodactylum tricornutum [PDF]

Journal of General Microbiology, 1958
SUMMARY: Phaeodactylum tricornutum Bohlin was isolated from a marine tank at Woods Hole, Mass., U.S.A. It appeared to be identical with the Plymouth strain of ‘Nitzschia closterium forma minutissima’. Cells were of two characteristic types, oval and fusiform, each of which remained constant for many cell divisions in clonal culture.
J C, LEWIN, R A, LEWIN, D E, PHILPOTT
openaire   +2 more sources

Dynamic Phaeodactylum tricornutum exometabolites shape surrounding bacterial communities [PDF]

New Phytologist, 2022
Summary Roles of different ecological classes of algal exometabolites in regulating microbial community composition are not well understood.
Vanessa Brisson, Courtney Swink, Jeffrey Kimbrel, Xavier Mayali, Ty Samo, Suzanne M. Kosina, Michael Thelen, Trent R. Northen, Rhona K. Stuart   +8 more
openaire   +3 more sources

Rapid Purification of Fucoxanthin from Phaeodactylum tricornutum

Molecules, 2022
Fucoxanthin is a natural marine xanthophyll and exhibits a broad range of biological activities. In the present study, a simple and efficient two-step method was used to purify fucoxanthin from the diatom, Phaeodactylum tricornutum. The crude pigment extract of fucoxanthin was separated by silica gel column chromatography (SGCC).
Xinjie Zhao, Liwei Gao, Xiangzhong Zhao
openaire   +3 more sources

Genome Properties of the Diatom Phaeodactylum tricornutum   [PDF]

Plant Physiology, 2002
Abstract Diatoms are a ubiquitous class of microalgae of extreme importance for global primary productivity and for the biogeochemical cycling of minerals such as silica. However, very little is known about diatom cell biology or about their genome structure.
SCALA S., CARELS N., FALCIATORE A., CHIUSANO, MARIA LUISA, BOWLER C.   +4 more
openaire   +3 more sources

Comparison of different lipid extraction procedures applied to three microalgal species

Energy Reports, 2020
The increase in the world’s energy demand has contributed to the emergence of new sustainable energy sources, such as microalgae, with their great potential to provide biofuels and other high value co-products for the food and health’s markets.
M. Gorgich, T.M. Mata, A.A. Martins, M. Branco-Vieira, N.S. Caetano   +4 more
doaj   +1 more source

Phylogenetic analysis and a review of the history of the accidental phytoplankter, Phaeodactylum tricornutum Bohlin (Bacillariophyta). [PDF]

PLoS ONE, 2018
The diatom Phaeodactylum tricornutum has been used as a model for cell biologists and ecologists for over a century. We have incorporated several new raphid pennates into a three gene phylogenetic dataset (SSU, rbcL, psbC), and recover Gomphonemopsis sp.
Jamal S M Sabir, Edward C Theriot, Schonna R Manning, Abdulrahman L Al-Malki, Mohammad A Khiyami, Areej K Al-Ghamdi, Mumdooh J Sabir, Dwight K Romanovicz, Nahid H Hajrah, Abdelfatteh El Omri, Robert K Jansen, Matt P Ashworth   +11 more
doaj   +1 more source