Results 11 to 20 of about 7,396 (223)

Optimizing CRISPR/Cas9 for the Diatom Phaeodactylum tricornutum [PDF]

Frontiers in Plant Science, 2018
CRISPR/Cas9 is a powerful tool for genome editing. We constructed an easy-to-handle expression vector for application in the model organism Phaeodactylum tricornutum and tested its capabilities in order to apply CRISPR/Cas9 technology for our purpose. In
Daniel Stukenberg, Stefan Zauner, Gianluca Dell’Aquila, Uwe G. Maier, Uwe G. Maier   +4 more
doaj   +4 more sources

Phaeodactylum tricornutum : a diatom cell factory [PDF]

Trends in Biotechnology, 2020
A switch from a petroleum-based to a biobased economy requires the capacity to produce both high-value low-volume and low-value high-volume products. Recent evidence supports the development of microalgae-based microbial cell factories with the objective
Rahul Kapoore, Butler, T., Kapoore, R.V., Vaidyanathan, S.   +3 more
core   +6 more sources

The Estimation of Biomass in Phaeodactylum Tricornutum Cultures [PDF]

Turkish Journal of Agriculture: Food Science and Technology, 2017
Phaeodactylum tricornutum is the microalgae that is known to produce lipid. In this study, it was aimed to estimate the microalgae Phaeodactylum tricornutum dry matter cultured in the conditions of nitrogen deficiency as a stress factor, in outdoor, in ...
Melis Çelik Güney, Oya Işık, Gökhan Tamer Kayaalp, Burcu Ak   +3 more
core   +4 more sources

NPF2 is involved in intracellular pH regulation and ion balance in the diatom Phaeodactylum tricornutum. [PDF]

New Phytol
Summary Cellular ion and pH homeostasis are crucial factors affecting and regulating metabolic reactions, protein stability, signalling and transport mechanisms. To balance intracellular ion concentrations, organisms need to constantly sense and respond to both the extracellular environment and cell compartments' requests.
Santin A, Russo MT, Croteau D, Ruggiero A, Russo Spena S, Morales de Los Rios L, Corratge-Faillie C, Brignone S, Lacombe B, Sanges R, Chiurazzi M, Ribera d'Alcalà M, Bailleul B, Falciatore A, Ferrante MI, Rogato A.   +15 more
europepmc   +2 more sources

Observations on Phaeodactylum tricornutum [PDF]

Journal of General Microbiology, 1958
SUMMARY: Phaeodactylum tricornutum Bohlin was isolated from a marine tank at Woods Hole, Mass., U.S.A. It appeared to be identical with the Plymouth strain of ‘Nitzschia closterium forma minutissima’. Cells were of two characteristic types, oval and fusiform, each of which remained constant for many cell divisions in clonal culture.
J C, LEWIN, R A, LEWIN, D E, PHILPOTT
openaire   +2 more sources

Plastidial ACP Δ9‐desaturase modulates EPA biosynthesis and TAG accumulation in Phaeodactylum tricornutum [PDF]

, 2021
International audienceThe unicellular marine diatom Phaeodactylum tricornutum accumulates up to 35% eicosapentaenoic acid (EPA, 20:5n3) and has been used as a model organism to study long chain polyunsaturated fatty acids (LC-PUFA) biosynthesis due to an
Richard, Smith, Sayanova, Olga, Nekrasov, V., Olga Sayanova, Marechal, Eric, Sayanova, O. V., Richard Smith, Napier, Johnathan A., Marechal, E., Gandini, Chiara, Smith, R., Gandini, C., Chiara Gandini, Johnathan A. Napier, Nekrasova, Vladimir, Jouhet, J., Juliette Jouhet, Napier, J. A., Eric Marechal, Vladimir Nekrasov, Jouhet, Juliette   +20 more
core   +1 more source

Genome Properties of the Diatom Phaeodactylum tricornutum   [PDF]

Plant Physiology, 2002
Abstract Diatoms are a ubiquitous class of microalgae of extreme importance for global primary productivity and for the biogeochemical cycling of minerals such as silica. However, very little is known about diatom cell biology or about their genome structure.
SCALA S., CARELS N., FALCIATORE A., CHIUSANO, MARIA LUISA, BOWLER C.   +4 more
openaire   +3 more sources

Phylogenetic analysis and a review of the history of the accidental phytoplankter, Phaeodactylum tricornutum Bohlin (Bacillariophyta). [PDF]

PLoS ONE, 2018
The diatom Phaeodactylum tricornutum has been used as a model for cell biologists and ecologists for over a century. We have incorporated several new raphid pennates into a three gene phylogenetic dataset (SSU, rbcL, psbC), and recover Gomphonemopsis sp.
Jamal S M Sabir, Edward C Theriot, Schonna R Manning, Abdulrahman L Al-Malki, Mohammad A Khiyami, Areej K Al-Ghamdi, Mumdooh J Sabir, Dwight K Romanovicz, Nahid H Hajrah, Abdelfatteh El Omri, Robert K Jansen, Matt P Ashworth   +11 more
doaj   +1 more source

Identification of Loci Enabling Stable and High-Level Heterologous Gene Expression

Frontiers in Bioengineering and Biotechnology, 2021
Efficient and reliable genome engineering technologies have yet to be developed for diatoms. The delivery of DNA in diatoms results in the random integration of multiple copies, quite often leading to heterogeneous gene activity, as well as host ...
Gilles Defrel, Nathalie Marsaud, Etienne Rifa, Frédéric Martins, Frédéric Martins, Fayza Daboussi, Fayza Daboussi   +6 more
doaj   +1 more source

Action Spectrum of Photoinhibition in the Diatom Phaeodactylum tricornutum [PDF]

Plant and Cell Physiology, 2017
Light-dependent electron transfer is necessary for photosynthesis, but light also damages PSII. Light-induced damage to PSII is called photoinhibition, and the damaging reactions of photoinhibition are still under debate. Diatoms possess an exotic combination of light-harvesting pigments, Chls a/c and fucoxanthin, making them an interesting platform ...
Tyystjärvi Esa, Havurinne Vesa
openaire   +2 more sources