Results 11 to 20 of about 8,719 (157)

Optimizing CRISPR/Cas9 for the Diatom Phaeodactylum tricornutum

Frontiers in Plant Science, 2018
CRISPR/Cas9 is a powerful tool for genome editing. We constructed an easy-to-handle expression vector for application in the model organism Phaeodactylum tricornutum and tested its capabilities in order to apply CRISPR/Cas9 technology for our purpose. In
Daniel Stukenberg, Stefan Zauner, Gianluca Dell’Aquila, Uwe G. Maier, Uwe G. Maier   +4 more
doaj   +2 more sources

Plastidial ACP Δ9‐desaturase modulates EPA biosynthesis and TAG accumulation in Phaeodactylum tricornutum [PDF]

, 2021
International audienceThe unicellular marine diatom Phaeodactylum tricornutum accumulates up to 35% eicosapentaenoic acid (EPA, 20:5n3) and has been used as a model organism to study long chain polyunsaturated fatty acids (LC-PUFA) biosynthesis due to an
Richard, Smith, Sayanova, Olga, Nekrasov, V., Olga Sayanova, Marechal, Eric, Sayanova, O. V., Richard Smith, Napier, Johnathan A., Marechal, E., Gandini, Chiara, Smith, R., Gandini, C., Chiara Gandini, Johnathan A. Napier, Nekrasova, Vladimir, Jouhet, J., Juliette Jouhet, Napier, J. A., Eric Marechal, Vladimir Nekrasov, Jouhet, Juliette   +20 more
core   +1 more source

Identification of Loci Enabling Stable and High-Level Heterologous Gene Expression

Frontiers in Bioengineering and Biotechnology, 2021
Efficient and reliable genome engineering technologies have yet to be developed for diatoms. The delivery of DNA in diatoms results in the random integration of multiple copies, quite often leading to heterogeneous gene activity, as well as host ...
Gilles Defrel, Nathalie Marsaud, Etienne Rifa, Frédéric Martins, Frédéric Martins, Fayza Daboussi, Fayza Daboussi   +6 more
doaj   +1 more source

Investigation of Metal Toxicity on Microalgae Phaeodactylum tricornutum, Hipersaline Zooplankter Artemia salina, and Jellyfish Aurelia aurita

Toxics, 2023
The escalating global anthropogenic activities associated with industrial development have led to the increased introduction of heavy metals (HMs) into marine environments through effluents.
Borja Mercado, Nuria Valero, Luis Roca-Pérez, Elena Bernabeu-Berni, Oscar Andreu-Sánchez   +4 more
doaj   +1 more source

Deciphering the Allosteric Process of Phaeodactylum tricornutum Aureochrome 1a LOV Domain [PDF]

, 2020
The conformational-driven allosteric protein diatom Phaeodactylum tricornutum aureochrome 1a (PtAu1a) di ers from other light-oxygen-voltage (LOV) proteins for its uncommon structural topology. The mechanism of signaling transduction in PtAu1a LOV domain
Francesco, Trozzi, Peng, Tao, Hao, Tian, Brian, Zoltowski   +3 more
core   +1 more source

Characterization of a trimeric light-harvesting complex in the diatom Phaeodactylum tricornutum built of FcpA and FcpE proteins [PDF]

, 2010
Fucoxanthin chlorophyll proteins (Fcps), the light-harvesting antennas of heterokont algae, are encoded by a multigene family and are highly similar with respect to their molecular masses as well as to their pigmentation, making it difficult to purify ...
Kroth, Peter G., Schmidt, Matthias, Büchel, Claudia, Weisheit, Wolfram, Joshi-Deo, Jidnyasa, Mittag, Maria, Gruber, Ansgar   +6 more
core   +1 more source

Biosynthesis of fucoxanthin and diadinoxanthin and function of initial pathway genes in Phaeodactylum tricornutum [PDF]

, 2012
The biosynthesis pathway to diadinoxanthin and fucoxanthin was elucidated in Phaeodactylum tricornutum by a combined approach involving metabolite analysis identification of gene function.
Sandmann, Gerhard, Steiger, Sabine, Büchel, Claudia, Breitenbach, Jürgen, Dambek, Michael, Eilers, Ulrike   +5 more
core   +1 more source

Transcriptome, Biochemical and Growth Responses of the Marine Phytoplankter Phaeodactylum Tricornutum Bohlin (Bacillariophyta) to Copepod Grazer Presence

Cellular Physiology and Biochemistry, 2018
Background/Aims: As a model organism for a pleiomorphic marine planktonic primary producer, Phaeodactylum tricornutum has been studied on a molecular level under diverse cultural conditions.
Si Li, Stefanie M.H. Ismar
doaj   +1 more source

Trypsin is a coordinate regulator of N and P nutrients in marine phytoplankton

Nature Communications, 2022
Using CRISPR-Cas9 mediated-knockout and overexpression analyses, this study shows that a trypsin in the diatom Phaeodactylum tricornutum promotes phosphorus uptake and inhibits nitrogen uptake but its expression is downregulated under nitrogen stress and
Yanchun You, Xueqiong Sun, Minglei Ma, Jiamin He, Ling Li, Felipe Wendt Porto, Senjie Lin   +6 more
doaj   +1 more source