Results 91 to 100 of about 5,332,729 (289)

Phase Noise Compensation for Nonlinearity-Tolerant Digital Subcarrier Systems With High-Order QAM

IEEE Photonics Journal, 2017
The fundamental penalty of subcarrier modulation (SCM) with independent subcarrier phase noise processing is estimated. It is shown that the fundamental signal-to-noise ratio (SNR) penalty related to poorer phase noise tolerance of decreased baudrate ...
M. P. Yankov, L. Barletta, D. Zibar
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Digital Phase Noise Compensation for DSCM-Based Superchannel Transmission System With Quantum Dot Passive Mode-Locked Laser

IEEE Photonics Journal, 2018
We propose a simplified digital phase noise compensation technique for a Nyquist pulse-shaped digital subcarrier multiplexed (DSCM) coherent optical transmission system, employing an optical frequency comb based on Quantum dot passive mode-locked laser ...
Govind Vedala, Maurice O'Sullivan, Rongqing Hui   +2 more
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Simplified Laser Frequency Noise Measurement Using the Delayed Self-Heterodyne Method

Photonics
Here, we report on a simplified laser frequency noise measurement technique employing an acousto-optic modulator, a delay line, and a real-time oscilloscope. The technique is a slight modification of the typical delayed heterodyne method.
Seán P. Ó Dúill, Liam P. Barry
doaj   +1 more source

Experimental Studies on the Noise Properties of the Harmonics From a Passively Mode-Locked Er-Doped Fiber Laser

IEEE Photonics Journal, 2019
We experimentally investigate the noise properties of a homemade 586 MHz mode-locked laser (MLL). The variation of the timing jitter versus the harmonic order is measured, which is consistent with the theoretical analyses.
Jiazheng Song, Xiaohong Hu, Hushan Wang, Tao Duan, Yishan Wang, Yuanshan Liu, Jianguo Zhang   +6 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Noise performance of frequency modulation Kelvin force microscopy

Beilstein Journal of Nanotechnology, 2014
Noise performance of a phase-locked loop (PLL) based frequency modulation Kelvin force microscope (FM-KFM) is assessed. Noise propagation is modeled step by step throughout the setup using both exact closed loop noise gains and an approximation known as “
Heinrich Diesinger, Dominique Deresmes, Thierry Mélin   +2 more
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source