Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Plasmonic Response to Liquid-Solid Phase Transition in Individual Gallium Nanoparticles. [PDF]
Horák M +6 more
europepmc +1 more source
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source
A thermo-responsive sol-to-gel phase transition hydrogel for sustained delivery of mesenchymal stem cell-derived exosomes. [PDF]
Liu L +7 more
europepmc +1 more source
Acute caffeine treatment protects the developing retina from ischemia‐induced cell death
Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento +6 more
wiley +1 more source
Nonporous hydrophobic organic crystals for carbon dioxide capture via chain-melting phase transition. [PDF]
Petrović A +11 more
europepmc +1 more source
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero +5 more
wiley +1 more source
Doping-induced magnetic phase transition enables all-electrical spin control in CrSBr. [PDF]
Zhao G +17 more
europepmc +1 more source
Pressure-Induced Structural Phase Transition in Gd<sub>2</sub>Ce<sub>2</sub>O<sub>7</sub> Oxide. [PDF]
Lv T +6 more
europepmc +1 more source
Visualization-guided growth of CsPbBr<sub>3</sub> microcrystals via water-induced phase transition. [PDF]
Zhong Y +9 more
europepmc +1 more source

