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Hydrolysis of native poly(hydroxybutyrate) granules (PHB), crystalline PHB, and artificial amorphous PHB granules by intracellular and extracellular depolymerases

International Journal of Biological Macromolecules, 1999
Native poly(hydroxybutyrate) (PHB) granules, purified PHB and artificial amorphous PHB granules were examined as putative substrates for hydrolysis by the intracellular depolymerase system of Rhodospirillum rubrum and the extracellular depolymerase of Pseudomonas lemoignei. The R.
J M, Merrick, R, Steger, D, Dombroski
openaire   +2 more sources

Polyhydroxybutyrate (PHB) biodegradation using bacterial strains with demonstrated and predicted PHB depolymerase activity

Applied Microbiology and Biotechnology, 2018
The biodegradation of polyhydroxybutyrate (PHB) has been broadly investigated, but studies typically focus on a single strain or enzyme and little attention has been paid to comparing the interaction of different PHB depolymerase (PhaZ)-producing strains with this biopolymer.
Diana Isabel, Martínez-Tobón   +3 more
openaire   +2 more sources

Improvement of polyhydroxybutyrate (PHB) plate-based screening method for PHB degrading bacteria using cell-grown amorphous PHB and recovered by sodium dodecyl sulfate (SDS)

International Journal of Biological Macromolecules, 2021
Poly(3-hydroxybutyrate) (PHB) is a biobased and biodegradable plastic. Considering the environmental issues of petroleum-based plastics, PHB is promising as it can be degraded in a relatively short time by bacteria to water and carbon dioxide. Substantial efforts have been made to identify PHB-degrading bacteria.
Sol Lee, Park   +10 more
openaire   +2 more sources

Biodegradability of Thermally Aged PHB, PHB-V, and PCL in Soil Compostage

Journal of Polymers and the Environment, 2004
The biodegradability of poly-β-hydroxybutyrate (PHB), poly-β-hydroxybutyrate-co-valerate (PHB-V) and poly-ɛ-caprolactone (PCL) were examined following thermal aging in an oven for 192, 425 and 600 h. Different temperatures, 100, 120 and 140°C for PHB and PHB-V and 30, 40 and 50oC for PCL were used to assess the influence of this parameter on ...
Derval dos Santos Rosa   +3 more
openaire   +1 more source

A convenient route to PHB macromonomers via anionically controlled moderate‐temperature degradation of PHB

Journal of Polymer Science Part A: Polymer Chemistry, 2010
AbstractThe degradation of poly(3‐hydroxybutyrate)s in homo‐ and heterogeneous mixtures with selected salts of organic and mineral acids was investigated. Nonvolatile degradation products, of processes conducted at moderate temperatures (150–170 °C), were analyzed using 1H NMR.
M. Kawalec   +4 more
openaire   +2 more sources

Controlling microbial PHB synthesis via CRISPRi

Applied Microbiology and Biotechnology, 2017
Microbial polyhydroxyalkanoates (PHA) are a family of biopolyesters with properties similar to petroleum plastics such as polyethylene (PE) or polypropylene (PP). Polyhydroxybutyrate (PHB) is the most common PHA known so far. Clustered regularly interspaced short palindromic repeats interference (CRISPRi), a technology recently developed to control ...
Dan, Li   +3 more
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Spherulitic Growth Rate of Blends of Polyhydroxybutyrate (PHB) with Oligomeric Atactic PHB-diol

Journal of Macromolecular Science, Part B, 2011
Spherulitic growth rates of blends of polyhydroxybutyrate (PHB) with oligomers of atactic R,S-PHB-OH (Mn = 610, 910, and 2670 g/mol), as well as atactic R,S-PHB (Mn = 30 000 g/mol), were investigated by polarized light optical microscopy in the range from 50°C to 110°C.
S. H. El-Taweel, B. Stoll
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The “PHB Depolymerase Inhibitor” of Paucimonas lemoignei Is a PHB Depolymerase

Biomacromolecules, 2002
A approximately 35 kDa protein that has been described to be secreted by Paucimonas lemoignei during growth on succinate and to inhibit hydrolysis of denatured (crystalline) poly(3-hydroxybutyrate) (dPHB) by extracellular PHB depolymerases of P. lemoignei (PHB depolymerase inhibitor (PDI)) was purified and characterized.
Simone, Reinhardt   +2 more
openaire   +2 more sources

Study on Synthesis of PHB by Moderate Halophile and Aqueous Extraction of PHB

Applied Mechanics and Materials, 2013
PHB (Poly-β-hydroxybutyrate) has been widely in the areas of industrial packaging, pharmacy and agriculture. Currently, its production cost, especially in extraction and purification are high. A moderate halophilic bacterium was used for PHB synthesis and an aqueous extraction method based on osmotic downshock was investigated in this paper to further ...
Qing Chen, Ling Hua Zhang
openaire   +1 more source

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