Genomic characterization of Phenylalanine ammonia lyase gene in buckwheat [PDF]
Phenylalanine Ammonia Lyase (PAL) gene which plays a key role in bio-synthesis of medicinally important compounds, Rutin/quercetin was sequence characterized for its efficient genomics application. These compounds possessing anti-diabetic and anti-cancer
Cantale, C. +5 more
core +6 more sources
Maize Phenylalanine Ammonia-Lyase Has Tyrosine Ammonia-Lyase Activity [PDF]
Abstract A full-length cDNA encoding phenylalanine ammonia-lyase (PAL) from Zea mays L. was isolated and the coding region was expressed in Escherichia coli as a C-terminal fusion to glutathione S-transferase. After purification by glutathione-Sepharose chromatography, the glutathione S-transferase moiety was cleaved off and the ...
J, Rösler +3 more
openaire +2 more sources
Erythrocytes as Carriers of Therapeutic Enzymes. [PDF]
Therapeutic enzymes are administered for the treatment of a wide variety of diseases. They exert their effects through binding with a high affinity and specificity to disease-causing substrates to catalyze their conversion to a non-noxious product, to ...
Bax, BE
core +1 more source
Phenylalanine Ammonia-lyase of Bamboo Shoots [PDF]
Phenylalanine ammonia-lyase (PAL) was purified from bamboo shoots (Bambusa oldhami Munro) through steps of DEAE-Sephacel column chromatography, Sepharose CL-6B gel filtration, and a second DEAE-Sephacel column chromatography. Polyacrylamide gel electrophoresis showed that the purified enzyme (Mr 360, 000) still contained two minor bands with much ...
Ru-Yin CHEN +2 more
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Fungal and Plant Phenylalanine Ammonia-lyase [PDF]
L-Phenylalanine is one of the essential amino acids that cannot be synthesized in mammals in adequate amounts to meet the requirements for protein synthesis. Fungi and plants are able to synthesize phenylalanine via the shikimic acid pathway. L-Phenylalanine, derived from the shikimic acid pathway, is used directly for protein synthesis in plants or ...
Hyun, Min Woo +3 more
openaire +2 more sources
Optimization of L-phenylalanine-ammonia-lyase liophilization [PDF]
The influence of freezing-thawing on the activity of recombinant L-phenylalanine-ammonia-lyase has been investigated. Conditions of liophilization of recombinant L-phenylalanine-ammonia-lyase at which enzyme loses about 10% of activity in a single freeze-thaw cycle are chosen. Dependence of cryoscopic temperature of enzyme from concentration of protein
O O, Babich, A Iu, Prosekov
openaire +2 more sources
A NOVEL PHENYLALANINE AMMONIA-LYASE FROM KANGIELLA KOREENSIS [PDF]
This study describes cloning of the gene encoding a novel phenylalanine ammonia-lyase from Kangiella koreensis (KkPAL) into pET19b expression vector. Optimization of protein expression and purification conditions yielded 15 mg pure soluble protein from ...
Adriana MARCOVICI +8 more
core +2 more sources
The expression of stlA in Photorhabdus luminescens is controlled by nutrient limitation [PDF]
Photorhabdus is a genus of Gram-negative entomopathogenic bacteria that also maintain a mutualistic association with nematodes from the family Heterorhabditis.
Bode, Helge Björn +3 more
core +8 more sources
Obstruction of Water Uptake in cut Chrysanthemum Stems after Dry Storage: Role of Wound-induced Increase in Enzyme Activities and Air Emboli [PDF]
Hydraulic conductance of cut chrysanthemum stems was lowered by the aspiration of air as well as by a wound-induced plant response. By measuring the hydraulic conductance of stem segments in which air could be introduced into and/or removed from the ...
Arevalo-Galarza, L., Meeteren, U., van
core +2 more sources
Nanogram amounts of salicylic acid produced by the rhizobacterium Pseudomonas aeruginosa 7NSK2 activate the systemic acquired resistance pathway in bean [PDF]
Root colonization by specific nonpathogenic bacteria can induce a systemic resistance in plants to pathogen infections. In bean, this kind of systemic resistance can be induced by the rhizobacterium Pseudomonas aeruginosa 7NSK2 and depends on the ...
Audenaert, Kris +5 more
core +2 more sources

