Results 11 to 20 of about 877,235 (257)

Elucidating the Phosphate Binding Mode of Phosphate-Binding Protein: The Critical Effect of Buffer Solution [PDF]

The Journal of Physical Chemistry B, 2018
Phosphate is an essential component of cell functions, and the specific transport of phosphorus into a cell is mediated by phosphate-binding protein (PBP). The mechanism of PBP-phosphate recognition remains controversial: on the basis of similar binding affinities at acidic and basic pHs, it is believed that the hydrogen network in the binding site is ...
Rui Qi, Zhifeng Jing, Chengwen Liu, Jean-Philip Piquemal, Kevin N. Dalby, Pengyu Ren   +5 more
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Phosphate binding sites identification in protein structures [PDF]

Nucleic Acids Research, 2010
Nearly half of known protein structures interact with phosphate-containing ligands, such as nucleotides and other cofactors. Many methods have been developed for the identification of metal ions-binding sites and some for bigger ligands such as carbohydrates, but none is yet available for the prediction of phosphate-binding sites.
Parca, L, Gherardini, PF, HELMER CITTERICH, MANUELA, AUSIELLO, GABRIELE   +3 more
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20S and 26S proteasome-binding proteins of the rabbit brain: A proteomic dataset

Data in Brief, 2021
Fractions of 26S and 20S proteasomes isolated from the rabbit brain by the method of salt fractionation (salt-induced precipitation) contain intrinsic proteasome proteins responsible for assembly of the core particle and regulatory particle of proteasome
Olga Buneeva, Arthur Kopylov, Svetlana Kaloshina, Victor Zgoda, Alexei Medvedev   +4 more
doaj   +1 more source

Identification of nucleotide-binding sites in protein structures: a novel approach based on nucleotide modularity. [PDF]

PLoS ONE, 2012
Nucleotides are involved in several cellular processes, ranging from the transmission of genetic information, to energy transfer and storage. Both sequence and structure based methods have been developed to predict the location of nucleotide-binding ...
Luca Parca, Pier Federico Gherardini, Mauro Truglio, Iolanda Mangone, Fabrizio Ferrè, Manuela Helmer-Citterich, Gabriele Ausiello   +6 more
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Phosphate binding sites prediction in phosphorylation-dependent protein–protein interactions

Bioinformatics, 2021
Abstract Motivation Phosphate binding plays an important role in modulating protein–protein interactions, which are ubiquitous in various biological processes. Accurate prediction of phosphate binding sites is an important but challenging task.
Zheng-Chang Lu, Fan Jiang, Yun-Dong Wu
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Mechanism of phosphate sensing and signaling revealed by rice SPX1-PHR2 complex structure

Nature Communications, 2021
SPX proteins sense phosphate levels in plant cells by binding to inositol polyphosphates (InsP) and suppressing the activity of PHR transcription factors. Here the authors show that when bound to InsP6, the rice SPX1 protein inhibits the activity of PHR2
Jia Zhou, Qinli Hu, Xinlong Xiao, Deqiang Yao, Shenghong Ge, Jin Ye, Haojie Li, Rujie Cai, Renyang Liu, Fangang Meng, Chao Wang, Jian-Kang Zhu, Mingguang Lei, Weiman Xing   +13 more
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Phosphate-binding Tag, a New Tool to Visualize Phosphorylated Proteins [PDF]

Molecular & Cellular Proteomics, 2006
We introduce two methods for the visualization of phosphorylated proteins using alkoxide-bridged dinuclear metal (i.e. Zn(2+) or Mn(2+)) complexes as novel phosphate-binding tag (Phos-tag) molecules. Both Zn(2+)- and Mn(2+)-Phos-tag molecules preferentially capture phosphomonoester dianions bound to Ser, Thr, and Tyr residues. One method is based on an
Kinoshita, Eiji, Kinoshita-Kikuta, Emiko, Takiyama, Kei, Koike, Tohru   +3 more
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Structural comparison of unconventional G protein YchF with heterotrimeric G protein and small G protein

Plant Signaling & Behavior, 2022
Guanine nucleotide-binding (G) proteins, namely, phosphate-binding (P) loop GTPases, play a critical role in life processes among different species. Based on the structural characteristics, G proteins can be divided into heterotrimeric G proteins, small ...
Maozhen Luo, Zhiwei Han, Guoye Huang, Rongfang Li, Yi Liu, Junjie Lu, Lin Liu, Rui Miao   +7 more
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Distinction between 2′- and 3′-Phosphate Isomers of a Fluorescent NADPH Analogue Led to Strong Inhibition of Cancer Cells Migration

Antioxidants, 2021
Specific inhibition of NADPH oxidases (NOX) and NO-synthases (NOS), two enzymes associated with redox stress in tumor cells, has aroused great pharmacological interest.
Raoul Manuel, Michelle de Souza Lima, Sébastien Dilly, Sylvain Daunay, Patricia Abbe, Elodie Pramil, Stéphanie Solier, Fabienne Guillaumond, Sarah-Simha Tubiana, Alexandre Escargueil, João Antonio Pêgas Henriques, Nathalie Ferrand, Irène Erdelmeier, Jean-Luc Boucher, Gildas Bertho, Israel Agranat, Stéphane Rocchi, Michèle Sabbah, Anny Slama Schwok   +18 more
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Contribution of protein phosphorylation to binding-induced folding of the SLBP–histone mRNA complex probed by phosphorus-31 NMR

FEBS Open Bio, 2014
Phosphorus-31 (31P) NMR can be used to characterize the structure and dynamics of phosphorylated proteins. Here, I use 31P NMR to report on the chemical nature of a phosphothreonine that lies in the RNA binding domain of SLBP (stem-loop binding protein).
Roopa Thapar
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