Restoration of phosphate transport by the phosphate-binding protein in spheroplasts of Escherichia coli [PDF]
Journal of Bacteriology, 1977 Reconstitution of phosphate transport in Escherichia coli was demonstrated. Conversion of E. coli K10 cells to spheroplasts decreased phosphate transport to about 2%. Addition of purified phosphate-binding protein at physiological levels to these spheroplasts caused a mean 14-fold increase in phosphate transport rate.
R G, Gerdes +2 more
openaire +2 more sources
Identification of CP12 as a Novel Calcium-Binding Protein in Chloroplasts
Plants, 2013 Calcium plays an important role in the regulation of several chloroplast processes. However, very little is still understood about the calcium fluxes or calcium-binding proteins present in plastids.
Agostinho Gomes Rocha, Ute C. Vothknecht
doaj +1 more source
Phosfinder: a web server for the identification of phosphate-binding sites on protein structures [PDF]
Nucleic Acids Research, 2011 Phosfinder is a web server for the identification of phosphate binding sites in protein structures. Phosfinder uses a structural comparison algorithm to scan a query structure against a set of known 3D phosphate binding motifs. Whenever a structural similarity between the query protein and a phosphate binding motif is detected, the phosphate bound by ...
Parca, L +4 more
openaire +4 more sources
Microbial Cell Factories, 2004 Sugar binding proteins and binders of intermediate sugar metabolites derived from microbes are increasingly being used as reagents in new and expanding areas of biotechnology.
Bhattacharya Sumana +6 more
doaj +1 more source
Control of the synthesis of alkaline phosphatase and the phosphate-binding protein in Escherichia coli [PDF]
Journal of Bacteriology, 1976 Using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunological techniques, we have compared the synthesis of the phoA protein (alkaline phosphatase) and the phoS protein (phosphate-binding protein) in response to the level of phosphate in the medium in different genetic backgrounds containing the known alkaline phosphatase control ...
G R, Willsky, M H, Malamy
openaire +2 more sources
Structural and molecular basis for phosphate recognition by SAR11 bacteria
mBioPhosphorus is a critical limiting nutrient that constrains the survival, growth, and reproduction of marine microorganisms in nutrient-limited ecosystems.
Wen-Jing Zhu +11 more
doaj +1 more source
Frontiers in Cellular and Infection Microbiology, 2015 Bacterial cell wall (CW) and extracellular (EC) proteins are often involved in interactions with extracellular matrix (ECM) proteins such as laminin (LN) and fibronectin (FN), which play important roles in adhesion and invasion.
Quan eLi +8 more
doaj +1 more source
Mycobacterium tuberculosis AtsG (Rv0296c), GlmU (Rv1018c) and SahH (Rv3248c) Proteins Function as the Human IL-8-Binding Effectors and Contribute to Pathogen Entry into Human Neutrophils. [PDF]
PLoS ONE, 2016 Mycobacterium tuberculosis is an extremely successful intracellular pathogen that has evolved a broad spectrum of pathogenic mechanisms that enable its manipulation of host defense elements and its survival in the hostile environment inside phagocytes ...
Bozena Dziadek +8 more
doaj +1 more source
Characterization and partial purification of phosphate‐binding proteins in Candida tropicalis
FEBS Letters, 1979 2.1. Obtaining the proteins Candida tropicalis (strain 101) was grown as in [ 11. Candida cells were incubated for 3 h in a medium lacking phosphate [l], in order to stimulate the ability of the cells to take up phosphate, and subjected to the modification [2] of the cold osmotic shock procedure [3].
Jeanjean, R., Fournier, N.
openaire +2 more sources
Eubacterial SpoVG homologs constitute a new family of site-specific DNA-binding proteins.
PLoS ONE, 2013 A site-specific DNA-binding protein was purified from Borrelia burgdorferi cytoplasmic extracts, and determined to be a member of the highly conserved SpoVG family.
Brandon L Jutras +6 more
doaj +1 more source