Eubacterial SpoVG homologs constitute a new family of site-specific DNA-binding proteins.
PLoS ONE, 2013 A site-specific DNA-binding protein was purified from Borrelia burgdorferi cytoplasmic extracts, and determined to be a member of the highly conserved SpoVG family.
Brandon L Jutras +6 more
doaj +1 more source
Dimerization misalignment in human glutamate-oxaloacetate transaminase variants is the primary factor for PLP release. [PDF]
PLoS ONE, 2018 The active form of vitamin B6, pyridoxal 5'-phosphate (PLP), plays an essential role in the catalytic mechanism of various proteins, including human glutamate-oxaloacetate transaminase (hGOT1), an important enzyme in amino acid metabolism.
Jesi Lee +4 more
doaj +1 more source
Structure Characterization of Escherichia coli Pseudouridine Kinase PsuK
Frontiers in Microbiology, 2022 Pseudouridine (Ψ) is one of the most abundant RNA modifications in cellular RNAs that post-transcriptionally impact many aspects of RNA. However, the metabolic fate of modified RNA nucleotides has long been a question. A pseudouridine kinase (PsuK) and a
Xiaojia Li +7 more
doaj +1 more source
Investigation of Structural Dynamics of Enzymes and Protonation States of Substrates Using Computational Tools. [PDF]
, 2016 This review discusses the use of molecular modeling tools, together with existing experimental findings, to provide a complete atomic-level description of enzyme dynamics and function.
Chang, Chia-En A +3 more
core +2 more sources
Phosphate transport in Pseudomonas aeruginosa. Involvement of a periplasmic phosphate-binding protein [PDF]
European Journal of Biochemistry, 1984 A binding protein for inorganic phosphate was purified to apparent homogeneity from the shock fluids of phosphate-limited Pseudomonas aeruginosa. The purified protein bound one molecule of phosphate per molecule of binding protein with an average Kd of 0.34 microM. Arsenate, pyrophosphate and polyphosphates up to 15 units long could inhibit the binding
K, Poole, R E, Hancock
openaire +2 more sources
FEBS Letters, EarlyView.Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi +5 more
wiley +1 more source
High-Affinity Phosphate-Binding Protein (PBP) For Phosphorous Recovery: Proof of Concept Using Recombinant \u3cem\u3eEscherichia coli\u3c/em\u3e [PDF]
, 2016 Phosphorus (P) is a critical, non-renewable nutrient; yet excess discharges can lead to eutrophication and deterioration of water quality. Thus, P removal from water must be coupled with P recovery to achieve sustainable P management. P-specific proteins
Ballent, Wendy, Mayer, Brooke, Yang, Yu
core +1 more source
Folding mechanisms steer the amyloid fibril formation propensity of highly homologous proteins [PDF]
, 2018 Significant advances in the understanding of the molecular determinants of fibrillogenesis can be expected from comparative studies of the aggregation propensities of proteins with highly homologous structures but different folding pathways.
Baglivo, I +18 more
core +2 more sources
Reciprocal control of viral infection and phosphoinositide dynamics
FEBS Letters, EarlyView.Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley +1 more source
Microbiology SpectrumPhosphorus, a vital macronutrient, often limits primary productivity in marine environments. Marine Synechococcus strains, including WH8102, rely on high-affinity phosphate-binding proteins (PstS) to scavenge inorganic phosphate in oligotrophic oceans ...
Pramita Ranjit +3 more
doaj +1 more source