Results 331 to 340 of about 16,465,333 (371)
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Purification of Rat 2′,3′‐Cyclic Nucleotide 3′‐Phosphodiesterase
Journal of Neurochemistry, 1981Abstract: 2′,3′‐Cyclic nucleotide 3′‐phosphodiesterase (CNP, EC 3.1.4.37) has been isolated from rat brain myelin by chromatography on successive columns of phenyl‐Sepharose CL‐4B, CM‐Sepharose CL‐6B, and 8‐(6‐aminohexyl) amino‐2′AMP‐Sepharose 4B. From 15 g of rat brain, approximately 400 μg of pure CNP was obtained, with a specific activity of 1.200 (
Terry Joe Sprinkle, Michael R. Wells
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2′, 3′‐cyclic nucleotide 3′ ‐phosphodiesterase in cerebrospinal fluid
Neurology, 1981The activity of the myelin-associated enzyme 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP) was assayed in the cerebrospinal fluid (CSF) of 107 neurologic patients by a new and sensitive fluorometric method. The activity of CNP was about 20 nmol per hour per milligram protein or 12 nmol per hour per milliliter CSF.
Howard S. Maker +3 more
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2′,3′-Cyclic Nucleotide 3′-Phosphodiesterase
19782′,3′-Cyclic nucleotide 3′-phosphodiesterase (CNPase, EC 3.1.4.37*) has been widely used for several years as a marker for the presence of myelin in the central nervous system, but no review is available on its application and limitation as a marker enzyme and there is no information on its role in myelin.
Neil Raymond Sims, P. R. Carnegie
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Cyclic 3′,5′-AMP phosphodiesterase of Neurosporacrassa
Biochemical and Biophysical Research Communications, 1973Abstract Cyclic 3′,5′-AMP (cAMP) phosphodiesterase activity can be demonstrated in extracts of Neurospora crassa . The activity is particulate, has a pH optimum of 7.4, and consists of two forms that have different cAMP binding constants. Methylxanthines, inorganic phosphate, and EDTA are inhibitors of the diesterase as are ATP, ADP, and 8 ...
Bruce Solomon, William A. Scott
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Structural and functional evolution of 2′,3′-cyclic nucleotide 3′-phosphodiesterase
Brain Research, 20162',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) is an abundant membrane-associated enzyme within the vertebrate myelin sheath. While the physiological function of CNPase still remains to be characterized in detail, it is known - in addition to its in vitro enzymatic activity - to interact with other proteins, small molecules, and membrane ...
Andrew E. Torda +5 more
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Cyclic 3′, 5′-AMP phosphodiesterase of rabbit aorta
Biochimica et Biophysica Acta (BBA) - Enzymology, 1975Cyclic AMP and cyclic GMP phosphodiesterase activities (3' : 5'-cyclic AMP 5'-nucleotidohydrolase, EC 3.1.4.17) were demonstrated in the isolated intima, media, and adventitia of rabbit aorta. The activity for cyclic AMP hydrolysis in the intima was 2.7-fold higher than that for cyclic GMP hydrolysis. The activity for cyclic AMP hydrolysis in the media
Takio Shimamoto +2 more
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Origin of brain 2′,3′-cyclic-nucleotide 3′-phosphodiesterase doublet
Neuroscience Letters, 1992The present study established that 2',3'-cyclic-nucleotide 3'-phosphodiesterase doublet common to mammalian brain originates from an alternative splicing. Peptides specific to the predicted larger translation product were synthesized and antisera against these peptides were prepared.
Junko Yamamoto +5 more
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A modified assay of 3′:5′-cyclic-AMP phosphodiesterase
Analytical Biochemistry, 1975Abstract A modification of the assay of cyclic nucleotide phosphodiesterase involving batch use of Dowex 1 anion exchange resin is described which allows for quantitative recovery of adenosine, guanosine, and their metabolites from the resin slurry. The assay described is suitable for use in crude preparations containing purine catabolizing enzymes ...
R. J. Boudreau, G.I. Drummond
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Immunohistochemical localization of 2′,3′-cyclic nucleotide 3′-phosphodiesterase in the retina
Brain Research, 1982Abstract Immunohistochemical localization of 2′,3′-cyclic nucleotide 3′-phosphodiesterase (EC 3.1.4.37) in the bovine retina is described. The immunohistochemically stained sections showed that 2′,3′-cyclic nucleotide 3′-phosphodiesterase in the retina is primarily localized in the outer and inner segments of photoreceptor cells.
Yasuo Takahashi +2 more
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Structure of mouse 2′,3′-cyclic-nucleotide 3′-phosphodiesterase gene
Biochemical and Biophysical Research Communications, 1989The mouse 2',3'-cyclic-nucleotide 3'-phosphodiesterase gene was isolated from a mouse gene library. Restriction endonuclease mapping and DNA sequencing analysis revealed that this gene is about 6 kb long and is separated into three exons by two introns. The transcription initiation site was identified.
Katsumi Monoh +3 more
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