Results 191 to 200 of about 8,567 (253)

Phosphoenolpyruvate carboxylase (PEPC) and PEPC-kinase (PEPC-k) isoenzymes in Arabidopsis thaliana: role in control and abiotic stress conditions

Planta, 2016
Arabidopsis ppc3 mutant has a growth-arrest phenotype and is affected in phosphate- and salt-stress responses, showing that this protein is crucial under control or stress conditions. Phosphoenolpyruvate carboxylase (PEPC) and its dedicated kinase (PEPC-k) are ubiquitous plant proteins implicated in many physiological processes.
Ana B. Feria, Nadja Bosch, Alfonso Sánchez, Ana I. Nieto-Ingelmo, Clara de la Osa, Cristina Echevarrı́a, Sofía García‐Mauriño, José A. Monreal   +7 more
semanticscholar   +5 more sources

Phosphorylation of phosphoenolpyruvate carboxykinase (PEPCK) and phosphoenolpyruvate carboxylase (PEPC) in the flesh of fruits

Plant Physiology and Biochemistry, 2016
This study determined whether phosphoenolpyruvate carboxykinase (PEPCK) and phosphoenolpyruvate carboxylase (PEPC) are phosphorylated in the flesh of a range of fruits. This was done by incubating fruit flesh with 32P[P] (where 32P[P] = 32PO43-), then PEPCK and PEPC were immunoprecipitated from extracts using specific antisera. The incorporation of 32P[
Robert P. Walker, Andrea Paoletti, Richard C. Leegood, Franco Famiani   +3 more
semanticscholar   +6 more sources

Expression of Maize Gene Encoding C4-Pyruvate Orthophosphate Dikinase (PPDK) and C4-Phosphoenolpyruvate Carboxylase (PEPC) in Transgenic Arabidopsis

Plant Molecular Biology Reporter, 2012
To improve the efficiency of CO2 fixation in C3 photosynthesis, C4-cycle genes were overexpressed in Arabidopsis plants. In this study, a full-length cDNA for pyruvate orthophosphate dikinase (PPDK) with 971 amino acids was isolated from Zea mays using LA-PCR.
Yumin Wang, Weigang Xu, Lin Hu, Lei Zhang, Yan Li, Xi-He Du   +5 more
semanticscholar   +4 more sources

Pyramiding expression of maize genes encoding phosphoenolpyruvate carboxylase (PEPC) and pyruvate orthophosphate dikinase (PPDK) synergistically improve the photosynthetic characteristics of transgenic wheat

Protoplasma, 2014
Using particle bombardment transformation, we introduced maize pepc cDNA encoding phosphoenolpyruvate carboxylase (PEPC) and ppdk cDNA encoding pyruvate orthophosphate dikinase (PPDK) into the C3 crop wheat to generate transgenic wheat lines carrying cDNA of pepc (PC lines), ppdk (PK lines) or both (PKC lines).
Huifang Zhang, Weigang Xu, Wang HuiWei, Lin Hu, Yan Li, XueLi Qi, Lei Zhang, Chun-xin Li, Hua Xia   +8 more
semanticscholar   +4 more sources

Phosphoenolpyruvate carboxylase regulation in C4‐PEPC‐expressing transgenic rice during early responses to drought stress

Physiologia Plantarum, 2016
Phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) has important functions in C4 photosynthesis and biosynthesis of intermediate metabolites. In this study, the drought resistance of C4‐PEPC‐expressing transgenic rice (Oryza sativa, line PC) plants was assessed using simulated drought conditions [i.e. polyethylene glycol (PEG)‐6000 treatment]. The dry
Xiaolong Liu, Xia Li, Chen Zhang, Chuan‐Chao Dai, Jiayu Zhou, Cheng‐Gang Ren, Jinfei Zhang   +6 more
semanticscholar   +5 more sources

Recombinant thermoactive phosphoenolpyruvate carboxylase (PEPC) from Thermosynechococcus elongatus and its coupling with mesophilic/thermophilic bacterial carbonic anhydrases (CAs) for the conversion of CO2 to oxaloacetate

Bioorganic & Medicinal Chemistry, 2015
With the continuous increase of atmospheric CO2 in the last decades, efficient methods for carbon capture, sequestration, and utilization are urgently required. The possibility of converting CO2 into useful chemicals could be a good strategy to both decreasing the CO2 concentration and for achieving an efficient exploitation of this cheap carbon source.
Sonia Del Prete, Viviana De Luca, Clemente Capasso, Claudiu T. Supuran, Vincenzo Carginale   +4 more
semanticscholar   +7 more sources

Exogenous ATP enhance signal response of suspension cells of transgenic rice (Oryza sativa L.) expressing maize C 4 -pepc encoded phosphoenolpyruvate carboxylase under PEG treatment

Plant Growth Regulation, 2016
The aim of this study was to analyze the biochemical and molecular responses of suspension cells of transgenic rice (Oryza sativa L.) over-expressing the maize C 4 -pepc gene encodes phosphoenolpyruvate carboxylase (PEPC) (PC line) to drought stress by exogenous ATP ...
Kaifu Huo, X. Li, Yafei He, Xinlei Wei, Weiguo Lu, Chao Zhao, C. L. Wang   +6 more
semanticscholar   +5 more sources

Extrinsic thermostabilization factors and thermodenaturation mechanisms for phosphoenolpyruvate carboxylase (PEPC) from an extremely thermophilic bacterium Rhodothermus obamensis

Journal of Fermentation and Bioengineering, 1997
The thermodenaturation of phosphoenolpyruvate carboxylase (PEPC) from an extremely thermophilic bacterium Rhodothermus obamensis, capable of growth at temperature up to 85°C, was probed at different denaturation temperatures by UV-visible absorption, fluorescence emission and 1-anilinonaphthalene-8-sulfonate (ANS) binding and renaturation was assessed ...
Ken Takai, Yoshihiko Sako, Aritsune Uchida   +2 more
semanticscholar   +4 more sources

Bioinformatic identification of phosphoenolpyruvate carboxylase (PEPC) gene family in Brassica napus

Genetic Resources and Crop Evolution
Iffat Shaheen, Muhammad Waseem, Sana Basharat, Pingwu Liu, Abdul Qayyum   +4 more
semanticscholar   +3 more sources

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Inhibition of the enzyme phosphoenolpyruvate-carboxylase (PEPC) by different pollutants

Talanta, 1997
The inhibitory effect of different pollutants on the enzyme phosphoenolpyruvate-carboxylase was investigated. For this enzyme, different procedures are described for determining the activity of the enzyme. Determination of the presence of the enzymatically-formed oxaloacetate was found to be more suitable for this purpose because of the higher ...
Kathrin Stein, Gerd Ohlenbusch
openaire   +4 more sources