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Phosphorylation of phosphoenolpyruvate carboxykinase (PEPCK) and phosphoenolpyruvate carboxylase (PEPC) in the flesh of fruits

Plant Physiology and Biochemistry, 2016
This study determined whether phosphoenolpyruvate carboxykinase (PEPCK) and phosphoenolpyruvate carboxylase (PEPC) are phosphorylated in the flesh of a range of fruits. This was done by incubating fruit flesh with 32P[P] (where 32P[P] = 32PO43-), then PEPCK and PEPC were immunoprecipitated from extracts using specific antisera. The incorporation of 32P[
Franco Famiani   +2 more
exaly   +4 more sources

Inhibition of the enzyme phosphoenolpyruvate-carboxylase (PEPC) by different pollutants

Talanta, 1997
The inhibitory effect of different pollutants on the enzyme phosphoenolpyruvate-carboxylase was investigated. For this enzyme, different procedures are described for determining the activity of the enzyme. Determination of the presence of the enzymatically-formed oxaloacetate was found to be more suitable for this purpose because of the higher ...
Kathrin Stein
exaly   +3 more sources

Drought tolerance and proteomics studies of transgenic wheat containing the maize C4 phosphoenolpyruvate carboxylase (PEPC) gene

Protoplasma, 2015
Enhancing drought tolerance of crops has been a great challenge in crop improvement. Here, we report the maize phosphoenolpyruvate carboxylase (PEPC) gene was able to confer drought tolerance and increase grain yield in transgenic wheat (Triticum aestivum L.) plants.
Fang Yuhui, Xueli Qi, Weigang Xu
exaly   +3 more sources

Pyramiding expression of maize genes encoding phosphoenolpyruvate carboxylase (PEPC) and pyruvate orthophosphate dikinase (PPDK) synergistically improve the photosynthetic characteristics of transgenic wheat

Protoplasma, 2014
Using particle bombardment transformation, we introduced maize pepc cDNA encoding phosphoenolpyruvate carboxylase (PEPC) and ppdk cDNA encoding pyruvate orthophosphate dikinase (PPDK) into the C3 crop wheat to generate transgenic wheat lines carrying cDNA of pepc (PC lines), ppdk (PK lines) or both (PKC lines).
Xueli Qi, Weigang Xu, Huiwei Wang
exaly   +3 more sources

Phosphoenolpyruvate carboxylase (PEPC) and PEPC-kinase (PEPC-k) isoenzymes in Arabidopsis thaliana: role in control and abiotic stress conditions

Planta, 2016
Arabidopsis ppc3 mutant has a growth-arrest phenotype and is affected in phosphate- and salt-stress responses, showing that this protein is crucial under control or stress conditions. Phosphoenolpyruvate carboxylase (PEPC) and its dedicated kinase (PEPC-k) are ubiquitous plant proteins implicated in many physiological processes.
Ana B, Feria   +7 more
openaire   +2 more sources

The remarkable diversity of plant PEPC (phosphoenolpyruvate carboxylase): recent insights into the physiological functions and post-translational controls of non-photosynthetic PEPCs

Biochemical Journal, 2011
PEPC [PEP (phosphoenolpyruvate) carboxylase] is a tightly controlled enzyme located at the core of plant C-metabolism that catalyses the irreversible β-carboxylation of PEP to form oxaloacetate and Pi. The critical role of PEPC in assimilating atmospheric CO2 during C4 and Crassulacean acid metabolism photosynthesis has been studied extensively.
Brendan, O'Leary   +2 more
openaire   +2 more sources

Studying allelic diversity and stress response of PEPC (Phosphoenolpyruvate carboxylase) in Norway spruce (Picea abies)

Chemosphere, 1998
Abstract A novel experimental approach in ecological genetics for analysing allelic diversity at an isoenzyme-gene-system is presented. This approach is based on the question how individual genetic diversity is implemented in adaptive processes towards stress.
A. Ipsen   +3 more
openaire   +1 more source

Phosphoenolpyruvate carboxylase regulation in C4‐PEPC‐expressing transgenic rice during early responses to drought stress

Physiologia Plantarum, 2016
Phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) has important functions in C4 photosynthesis and biosynthesis of intermediate metabolites. In this study, the drought resistance of C4‐PEPC‐expressing transgenic rice (Oryza sativa, line PC) plants was assessed using simulated drought conditions [i.e. polyethylene glycol (PEG)‐6000 treatment]. The dry
Xiaolong, Liu   +6 more
openaire   +2 more sources

Extrinsic thermostabilization factors and thermodenaturation mechanisms for phosphoenolpyruvate carboxylase (PEPC) from an extremely thermophilic bacterium Rhodothermus obamensis

Journal of Fermentation and Bioengineering, 1997
The thermodenaturation of phosphoenolpyruvate carboxylase (PEPC) from an extremely thermophilic bacterium Rhodothermus obamensis, capable of growth at temperature up to 85°C, was probed at different denaturation temperatures by UV-visible absorption, fluorescence emission and 1-anilinonaphthalene-8-sulfonate (ANS) binding and renaturation was assessed ...
Ken Takai   +2 more
openaire   +1 more source

Physiological characteristics and metabolomics of transgenic wheat containing the maize C4 phosphoenolpyruvate carboxylase (PEPC) gene under high temperature stress

Protoplasma, 2016
In this paper, two transgenic wheat lines, PC27 and PC51, containing the maize PEPC gene and its wild-type (WT) were used as experimental material to study the effects of high temperature on their photosynthetic physiological characteristics and metabolome.
Xueli, Qi   +8 more
openaire   +2 more sources

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