Results 1 to 10 of about 24,002 (153)

Multiphoton Phosphorescence Quenching Microscopy Reveals Kinetics of Tumor Oxygenation during Antiangiogenesis and Angiotensin Signaling Inhibition. [PDF]

Clin Cancer Res, 2022
Abstract Purpose: The abnormal function of tumor blood vessels causes tissue hypoxia, promoting disease progression and treatment resistance. Although tumor microenvironment normalization strategies can alleviate hypoxia globally, how local oxygen levels change is not known because of the ...
Martin JD, Lanning RM, Chauhan VP, Martin MR, Mousa AS, Kamoun WS, Han HS, Lee H, Stylianopoulos T, Bawendi MG, Duda DG, Brown EB, Padera TP, Fukumura D, Jain RK.   +14 more
europepmc   +4 more sources

Interstitial Po₂ Determination by Phosphorescence Quenching Microscopy

Microcirculation, 2002
ABSTRACTObjective: This study introduces the technique of microinjection of phosphor probe into skeletal muscle tissue to determine oxygen tension (Po2) in the interstitium by phosphorescence quenching microscopy.Method: The spinotrapezius muscle of Wistar‐Kyoto rats weighing 240–280 g was surgically isolated and underwent the microinjection procedure.
Lane M, Smith, Aleksander S, Golub, Roland N, Pittman   +2 more
openaire   +3 more sources

A mathematical model of tissue axial and radial diffusion in the microvasculature for intravascular microscopy and phosphorescence quenching data

Computers in Biology and Medicine
This study aims to extend earlier Krogh Cylinder Models of an oxygen profile by considering axial diffusion and analytically solving Fick's Law Partial Differential Equation with novel boundary conditions via the separation of variables. We next prospectively collected a total of 20 animals, which were randomly assigned to receive either fresh or two ...
Vinay P. Jani, Vivek P. Jani, Carlos Munoz, Pedro Cabrales   +3 more
openaire   +4 more sources

Dynamics of PO2 and VO2 in resting and contracting rat spinotrapezius muscle

Frontiers in Physiology, 2023
This study examined changes in interstitial PO2, which allowed calculation of VO2 during periods of rest, muscle contraction and recovery using an in situ rat spinotrapezius muscle preparation.
Aleksander S. Golub, Aleksander S. Golub, Bjorn K. Song, Bjorn K. Song, William H. Nugent, William H. Nugent, Roland N. Pittman   +6 more
doaj   +2 more sources

Rate of oxygen disappearance from arterioles in rat mesentery measured with scanning phosphorescence quenching microscopy (PQM)

The FASEB Journal, 2010
The oxygen disappearance curve (ODC) recorded in an arteriole after rapid arrest of blood flow reflects the complex interaction between the HbO 2 dissociation curve and the kinetics of tissue respiration.
Aleksander Sergeevich Golub, Bjorn Kyungsuck Song, Roland Natan Pittman   +2 more
openaire   +2 more sources

Impact of accumulated oxygen depletion (AOD) on measurements of oxygen tension (PO2) using phosphorescence quenching microscopy (PQM)

The FASEB Journal, 2008
PQM is a powerful method to measure PO 2 in microvessels. However, its application to stationary tissue and fluids has never been substantiated. Multiple flash excitations applied in PQM to the same sampled volume produce AOD and distort the measured PO 2
Aleksander Sergeevich Golub, Roland Nathan Pittman   +1 more
openaire   +2 more sources

Oxygen dependence of respiratory rate (VO2) in rat spinotrapezius muscle in situ measured with phosphorescence quenching microscopy (PQM)

The FASEB Journal, 2012
Objective The sensitivity of isolated cells to PO2 is described by Hill's equation with a low Km (< 5 torr). Cells in intact tissues are more sensitive to O2 deficiency. To study the oxygen dependence of VO2 in muscle fibers we developed a method to register and interpret O2 disappearance curves (ODC).
Aleksander S Golub, Roland N Pittman
openaire   +2 more sources

Rationalizing the Unexpected Inefficiencies of 3D Porphyrin‐MOFs as Singlet Oxygen Photosensitizers Against Botrytis cinerea

Advanced Materials Interfaces
Porphyrin‐based MOFs (PMOFs) are known to be efficient photocatalysts and singlet oxygen (1O2) photosensitizers (PS) in solution, but their application in agriculture and food protection is practically unknown.
Océane Boudreau, Paul Asselin, Seyed Mehrzad Sajjadinezhad, Paul‐Ludovic Karsenti, Peter Moffett, Kamal Bouarab, Pierre D. Harvey   +6 more
doaj   +2 more sources

Gavage approach to oxygen supplementation with oxygen therapeutic Ox66™ in a hypoventilation rodent model of respiratory distress

Artificial Cells, Nanomedicine, and Biotechnology, 2021
Acute respiratory distress syndrome (ARDS) features pulmonary dysfunction capable of causing life-threatening hypoxaemia. Ventilation and hyperoxic therapies force oxygen through dysfunctional alveoli but risk exacerbating damage. Ox66™ is an ingestible,
William H. Nugent, Danuel A. Carr, Rosa MacBryde, Erica D. Bruce, Bjorn K. Song   +4 more
doaj   +1 more source

Systemic and microvascular comparison of Lactated Ringer’s solution, VIR-HBOC, and alpha-alpha crosslinked haemoglobin-based oxygen carrier in a rat 10% topload model

Artificial Cells, Nanomedicine, and Biotechnology, 2020
Medical support for traumatic haemorrhage is lacking for far-forward combat units. VIR-HBOC (haemoglobin-based oxygen carrier) is a novel biological therapeutic under development as a field-stable resuscitation fluid.
Bjorn K. Song, W. Richard Light, Kim D. Vandegriff, Joe Tucker, William H. Nugent   +4 more
doaj   +1 more source