Results 31 to 40 of about 37,163 (226)

Silencing of NAMPT leads to up-regulation of insulin receptor substrate 1 gene expression in U87 glioma cells

Endocrine Regulations, 2020
Objective. The aim of the present study was to investigate the effect of adipokine NAMPT (nicotinamide phosphoribosyltransferase) silencing on the expression of genes encoding IRS1 (insulin receptor substrate 1) and some other proliferation related ...
Tsymbal Daria O., Minchenko Dmytro O., Luzina Olena Y., Riabovol Olena O., Danilovskyi Serhiy V., Minchenko Oleksandr H.   +5 more
doaj   +1 more source

NAD- and NADPH-Contributing Enzymes as Therapeutic Targets in Cancer: An Overview

Biomolecules, 2020
Actively proliferating cancer cells require sufficient amount of NADH and NADPH for biogenesis and to protect cells from the detrimental effect of reactive oxygen species.
Alvinsyah Adhityo Pramono, Gulam M. Rather, Herry Herman, Keri Lestari, Joseph R. Bertino   +4 more
doaj   +1 more source

A Versatile Continuous Fluorometric Enzymatic Assay for Targeting Nicotinate Phosphoribosyltransferase

Molecules, 2023
The maintenance of a proper NAD+ pool is essential for cell survival, and tumor cells are particularly sensitive to changes in coenzyme levels. In this view, the inhibition of NAD+ biosynthesis is considered a promising therapeutic approach.
Gabriele Minazzato, Elisa Marangoni, Carlo Fortunato, Riccardo Petrelli, Loredana Cappellacci, Fabio Del Bello, Leonardo Sorci, Massimiliano Gasparrini, Francesco Piacente, Santina Bruzzone, Nadia Raffaelli   +10 more
doaj   +1 more source

Recurrent 2,8-Dihydroxyadenine Crystalline Nephropathy in Renal Allograft

Annals of Internal Medicine: Clinical Cases
Adenine phosphoribosyltransferase deficiency is a rare cause of crystalline kidney disease. We present a case of end-stage kidney disease secondary to crystal deposition from adenine phosphoribosyltransferase deficiency with immediate recurrence of the ...
Farid Arman, Russell John Crew, Michael Barry Stokes, Jai Radhakrishnan   +3 more
doaj   +1 more source

NAD+ metabolism controls inflammation during senescence

Molecular & Cellular Oncology, 2019
We have recently discovered that nicotinamide adenine dinucleotide metabolism controls the pro-inflammatory senescence-associated secretory phenotype during cellular senescence.
Timothy Nacarelli, Rugang Zhang
doaj   +1 more source

Extracellular nicotinate phosphoribosyltransferase binds Toll like receptor 4 and mediates inflammation

Nature Communications, 2019
The enzyme nicotinate phosphoribosyltransferase (NAPRT) mediates the rate-limiting step in NAD salvage pathway starting from nicotinic acid. Here the authors show that NAPRT can be detected extracellularly, binds to Toll like receptor 4, and activates NF-
Antonella Managò, Valentina Audrito, Francesca Mazzola, Leonardo Sorci, Federica Gaudino, Katiuscia Gizzi, Nicoletta Vitale, Danny Incarnato, Gabriele Minazzato, Alice Ianniello, Antonio Varriale, Sabato D’Auria, Giulio Mengozzi, Gianfranco Politano, Salvatore Oliviero, Nadia Raffaelli, Silvia Deaglio   +16 more
doaj   +1 more source

Anticancer sensitivities and biological characteristics of HCT116 cells resistant to the selective poly(ADP‐ribose) glycohydrolase inhibitor

FEBS Open Bio, EarlyView.
We analyzed alterations of PAR metabolism‐related proteins in PARG inhibitor‐resistant HCT116RPDD cells. Although PARG levels remained unchanged, HCT116RPDD cells exhibited reduced PARP1 and ARH3 expression and elevated PAR levels. Interestingly, HCT116RPDD cells exhibited slightly elevated intracellular NAD+/NADH and ATP levels. Our findings suggest a
Kaede Tsuda, Yoko Ogino, Akira Sato
wiley   +1 more source

Enhancing the anticancer effect of NAMPT inhibition by targeting NAPRT in breast cancer cells

Advances in Cancer Biology - Metastasis
Introduction: Two pathways in the synthesis of NAD, catalyzed by the key enzymes nicotinamide phosphoribosyltransferase (NAMPT) and nicotinate phosphoribosyltransferase (NAPRT), support constant survival, growth, and propagation of cancer cells.
Zahra Gharedaghi, Parichehr Mehrafshar, Soha Mohammadi, Masoumeh Tavakoli-Yaraki, Pegah Golpour, Mitra Nourbakhsh, Nima Taghizadeh   +6 more
doaj   +1 more source

A New 3D Colon on a Chip to Decipher the Influence of Mechanical Forces on the Physiological Cellular Ecosystem

Advanced Healthcare Materials, EarlyView.
To dissect how mechanical forces influence intestinal physiology, we developed a stretchable 3D colon‐on‐chip that integrates tunable topography, stiffness and peristalsis‐like motion within a physiologically relevant microenvironment. We showed that stretching is a dominant factor governing epithelial behavior, markedly enhancing proliferation and ...
Moencopi Bernheim‐Dennery, Lauriane Gérémie, Julie Brun, Lucas Chassatte, Giacomo Gropplero, Réda Bouras, Jieun Choo, Bertrand Cinquin, Alba Marcellan, Danijela Matic Vignjevic, Stéphanie Descroix   +10 more
wiley   +1 more source

Dihydroxyadenine stone with adenine phosphoribosyltransferase deficiency: A case report

Indian Journal of Urology, 2017
Adenine phosphoribosyltransferase (APRT) deficiency is a rare autosomal recessive error of purine metabolism resulting in the generation of 2,8-dihydroxyadenine (DHA), a highly insoluble metabolite of adenine, which can cause radiolucent urolithiasis ...
Pramod Krishnappa, Venkatesh Krishnamoorthy, Kiran Krishne Gowda   +2 more
doaj   +1 more source