Results 71 to 80 of about 3,382 (177)
Journal of the Science of Food and Agriculture, EarlyView.Abstract BACKGROUND Alphitobius diaperinus, the lesser mealworm, is an edible insect considered safe for human consumption; however, its proteins show documented cross‐reactivity with crustacean and house dust mite (HDM) allergens, raising potential concerns for allergic populations.
Michelle M Epstein +8 more
wiley +1 more source
Polynucleotide phosphorylases from thermophiles [PDF]
A purification procedure was developed for Eschericia coli polynucleotide phosphorylase, and subsequently applied to polynucleotide phosphorylases from Thermus aquaticus and Bacillus stearothermophilus.
Wood, John N. C.
core
Ion Activation Methods for Top‐Down Proteomics
Mass Spectrometry Reviews, EarlyView.ABSTRACT Mass spectrometry (MS) has emerged as a premier method used to characterize the sequences of proteins. Top‐down proteomics aims to capture the multiple sources of structural diversity reflected in proteins, such as those that arise from alternative RNA splicing events or the addition of post‐translational modifications. Tandem MS (i.e., MS/MS)
Jada N. Walker, Jennifer S. Brodbelt
wiley +1 more source
Discovery of new glycoside-phosphorylases for oligosaccharide synthesis
, 2020 Les glycoside-phosphorylases sont des enzymes actives sur les hydrates de carbone (CAZymes), capables de catalyser à la fois la dégradation des glycosides en utilisant le phosphate inorganique pour rompre les liaisons osidiques, et leur synthèse en ...
Li, Ao
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Metabolic buffering restricts phenotype switching in melanoma
The EMBO JournalThe impact of the metabolic microenvironment on epigenetically plastic cancer cells underpins phenotypic heterogeneity, a major cause of metastasis and therapy resistance.
Ana Ramírez-Sánchez +18 more
doaj +1 more source
PLoS ONE, 2014 The glycoside hydrolase family (GH) 65 is a family of inverting phosphorylases that act on α-glucosides. A GH65 protein (Bsel_2816) from Bacillus selenitireducens MLS10 exhibited inorganic phosphate (Pi)-dependent hydrolysis of kojibiose at the rate of 0.
Takanori Nihira +4 more
doaj +1 more source
Mass Spectrometry Reviews, EarlyView.ABSTRACT The use of MALDI mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this comprehensive review is the twelfth update of the original article published in 1999 and brings coverage of the literature to the end of 2024.
David J. Harvey
wiley +1 more source
, 2020 Glycoside hydrolases and phosphorylases are two major classes of enzymes responsible for the cleavage of glycosidic bonds. Here we show that two GH84 O-GlcNAcase enzymes can be converted to efficient phosphorylases by a single point mutation. Noteworthy,
Lluís Raich (2542087) +8 more
core +1 more source
Andrology, EarlyView.ABSTRACT Background Mammalian sperm need to undergo hyperactivation, capacitation, and acrosome reaction to achieve fertilization competence. However, the female reproductive tract presents contrasting metabolic environments. The uterus is glucose‐rich, and the oviduct is lactate‐rich and glucose‐limited.
Md Faizul Hossain Miraz +5 more
wiley +1 more source
Is there a role for cannabidiol in obesity, metabolic syndrome and binge eating?
British Journal of Pharmacology, EarlyView.Cannabidiol (CBD) is one of the most abundant phytocannabinoids isolated from the Cannabis sativa plant. CBD is a lipophilic, non‐intoxicating substance that differently from Δ9‐tetrahydrocannabinol (Δ9‐THC) does not present the typical profile of a drug of abuse.
Luca Botticelli +7 more
wiley +1 more source