Results 1 to 10 of about 62 (58)

Strained Conformations of Nucleosides in Active Sites of Nucleoside Phosphorylases

Biomolecules, 2020
Nucleoside phosphorylases catalyze the reversible phosphorolysis of nucleosides to heterocyclic bases, giving α-d-ribose-1-phosphate or α-d-2-deoxyribose-1-phosphate. These enzymes are involved in salvage pathways of nucleoside biosynthesis. The level of
Irina A Il'Icheva, K M Polyakov, Sergey N Mikhailov   +2 more
exaly   +3 more sources

Enzymatic synthesis and phosphorolysis of 4(2)-thioxo- and 6(5)-azapyrimidine nucleosides by E. coli nucleoside phosphorylases

Beilstein Journal of Organic Chemistry, 2016
The trans-2-deoxyribosylation of 4-thiouracil (4SUra) and 2-thiouracil (2SUra), as well as 6-azauracil, 6-azathymine and 6-aza-2-thiothymine was studied using dG and E.
Frank Seela, Igor A Mikhailopulo
exaly   +2 more sources

The chemoenzymatic synthesis of clofarabine and related 2′-deoxyfluoroarabinosyl nucleosides: the electronic and stereochemical factors determining substrate recognition by E. coli nucleoside phosphorylases

Beilstein Journal of Organic Chemistry, 2014
Two approaches to the synthesis of 2-chloro-9-(2-deoxy-2-fluoro-β-D-arabinofuranosyl)adenine (1, clofarabine) were studied. The first approach consists in the chemical synthesis of 2-deoxy-2-fluoro-α-D-arabinofuranose-1-phosphate (12a, 2FAra-1P) via ...
Ilja V Fateev, Irina D Konstantinova, Frank Seela   +2 more
exaly   +2 more sources

Bacterial Purine Nucleoside Phosphorylases from Mesophilic and Thermophilic Sources: Characterization of Their Interaction with Natural Nucleosides and Modified Arabinofuranoside Analogues

Biomolecules
The enzymatic synthesis of nucleoside derivatives is an important alternative to multi-step chemical methods traditionally used for this purpose. Despite several undeniable advantages of the enzymatic approach, there are a number of factors limiting its ...
Irina A Bychek, Anastasia A Zenchenko, Maria A Kostromina   +2 more
exaly   +3 more sources

Synthesis of 5-oxymethyl-1,2,4-triazole-3-carboxamides

Тонкие химические технологии, 2022
Objectives. A key step in the synthesis of natural nucleoside analogs is the formation of a glycosidic bond between the carbohydrate fragment and the heterocyclic base. Glycosylation methods differ in terms of regio- and stereoselectivity.
L. E. Grebenkina, A. N. Prutkov, A. V. Matveev, M. V. Chudinov   +3 more
doaj   +1 more source

SOME RECENT FINDINGS IN THE BIOTECHNOLOGY OF BIOLOGICALLY IMPORTANT NUCLEOSIDES [PDF]

Biotechnologia Acta, 2013
Some recent findings in the biotechnology of biologically important nucleosides will be discussed, viz., (i) a new strategy of the cascade one-pot transformation of D-pentoses into nucleosides based on the extension and deepening of the knowledge of the ...
A. Mikhailopulo, A. I. Miroshnikov
doaj   +1 more source

Efficient Biocatalytic Synthesis of Dihalogenated Purine Nucleoside Analogues Applying Thermodynamic Calculations

Molecules, 2020
The enzymatic synthesis of nucleoside analogues has been shown to be a sustainable and efficient alternative to chemical synthesis routes. In this study, dihalogenated nucleoside analogues were produced by thermostable nucleoside phosphorylases in ...
Heba Yehia, Sarah Westarp, Viola Röhrs, Felix Kaspar, Robert T. Giessmann, Hendrik F.T. Klare, Katharina Paulick, Peter Neubauer, Jens Kurreck, Anke Wagner   +9 more
doaj   +1 more source

Can Crystal Symmetry and Packing Influence the Active Site Conformation of Homohexameric Purine Nucleoside Phosphorylases?

Croatica Chemica Acta, 2016
It is generaly believed that enzymes retain most of their functionality in the crystal form due to the large solvent content of protein crystals. This is facilitated by the fact that their natural environment in solution is not too far from the one found
Marija Luić, Zoran Štefanić
doaj   +1 more source

Ethenoguanines undergo glycosylation by nucleoside 2'-deoxyribosyltransferases at non-natural sites. [PDF]

PLoS ONE, 2014
Deoxyribosyl transferases and functionally related purine nucleoside phosphorylases are used extensively for synthesis of non-natural deoxynucleosides as pharmaceuticals or standards for characterizing and quantitating DNA adducts.
Wenjie Ye, Debamita Paul, Lina Gao, Jolita Seckute, Ramiah Sangaiah, Karupiah Jayaraj, Zhenfa Zhang, P Alexandre Kaminski, Steven E Ealick, Avram Gold, Louise M Ball   +10 more
doaj   +1 more source

Insights into phosphate cooperativity and influence of substrate modifications on binding and catalysis of hexameric purine nucleoside phosphorylases. [PDF]

PLoS ONE, 2012
The hexameric purine nucleoside phosphorylase from Bacillus subtilis (BsPNP233) displays great potential to produce nucleoside analogues in industry and can be exploited in the development of new anti-tumor gene therapies.
Priscila O de Giuseppe, Nadia H Martins, Andreia N Meza, Camila R dos Santos, Humberto D'Muniz Pereira, Mario T Murakami   +5 more
doaj   +1 more source