Results 11 to 20 of about 38,543 (124)

Can Crystal Symmetry and Packing Influence the Active Site Conformation of Homohexameric Purine Nucleoside Phosphorylases? [PDF]

Croatica Chemica Acta, 2016
It is generaly believed that enzymes retain most of their functionality in the crystal form due to the large solvent content of protein crystals. This is facilitated by the fact that their natural environment in solution is not too far from the one found
Marija Luić, Zoran Štefanić
doaj   +2 more sources

Biosynthesis of Arabinoside from Sucrose and Nucleobase via a Novel Multi-Enzymatic Cascade [PDF]

Biomolecules
Arabinoside and derived nucleoside analogs, a family of nucleoside analogs, exhibit diverse typically biological activities and are widely used as antibacterial, antiviral, anti-inflammatory, antitumor, and other drugs in clinical and preclinical trials.
Yuxue Liu, Erchu Yang, Xiaojing Zhang, Xiaobei Liu, Xiaoting Tang, Zhenyu Wang, Hailei Wang   +6 more
doaj   +2 more sources

Efficient whole-cell biocatalytic synthesis of 2′-deoxy-2′-fluoroadenosine, a key building block for nucleic acid drugs [PDF]

Synthetic and Systems Biotechnology
2′-Deoxy-2′-fluoroadenosine (2′-F-dA) is a nucleoside analogue used as a key building block for oligonucleotide drugs. It can be biosynthesized from a low-cost 2′-deoxy-2′-fluorouridine via one-pot transglycosylation catalyzed by a thymidine ...
Dandan Feng, Tianshu Zhang, Guan Zhou, Yanli Cao, Quan Luo, Huifang Xu, Xuefeng Lu   +6 more
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Nucleotide Metabolism in Health and Disease. [PDF]

MedComm (2020)
Nucleotide metabolism, including de novo synthesis, salvage pathways, and catabolism, when dysregulated contributes to cancer, immune disorders, metabolic and urological diseases, and radiation injury. Metabolites such as adenosine, cGAMP, NAD, and cAMP and enzymes like RNR are promising therapeutic targets and biomarkers.
Zhao X, Zeng Y, Xu Y, Rao S, Khouchani M, Soummane H, Li J, Liu R.   +7 more
europepmc   +2 more sources

Structural and functional insights into Pseudomonas aeruginosa ApaH, a diadenosine tetraphosphatase crucial for bacterial virulence. [PDF]

Protein Sci
Abstract Infections by Pseudomonas aeruginosa are a major cause of severe morbidity and mortality in immunocompromised patients and people with cystic fibrosis, largely due to the pathogen's ability to resist antibiotic treatment and to deploy multiple virulence strategies that promote persistence in the host.
Pistoia G, Cervoni M, Catalano F, Troilo F, Guidi F, Comparini E, Mignogna G, Travaglini-Allocatelli C, Giuffrè A, Coluccia A, Imperi F, Di Matteo A, Giardina G.   +12 more
europepmc   +2 more sources

Comparative Analysis of Enzymatic Transglycosylation Using E. coli Nucleoside Phosphorylases: A Synthetic Concept for the Preparation of Purine Modified 2′-Deoxyribonucleosides from Ribonucleosides [PDF]

Int J Mol Sci, 2022
A comparative analysis of the transglycosylation conditions catalyzed by E. coli nucleoside phosphorylases, leading to the formation of 2′-deoxynucleosides, was performed. We demonstrated that maximal yields of 2′-deoxynucleosides, especially
Drenichev MS, Oslovsky VE, Zenchenko AA, Danilova CV, Varga MA, Esipov RS, Lykoshin DD, Alexeev CS.   +7 more
europepmc   +2 more sources

Commonality of Mechanism in Glycoside Hydrolases, Nucleoside Hydrolases, and Phosphorylases: Importance of Side-Chain Conformation Preorganization [PDF]

JACS Au
Po-Sen Tseng, Jonathan C. K. Quirke, W. Jonathan Lin, David Crich   +3 more
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The crystal structure of Streptococcus pyogenes uridine phosphorylase reveals a distinct subfamily of nucleoside phosphorylases. [PDF]

Biochemistry, 2011
Uridine phosphorylase (UP), a key enzyme in the pyrimidine salvage pathway, catalyzes the reversible phosphorolysis of uridine or 2'-deoxyuridine to uracil and ribose 1-phosphate or 2'-deoxyribose 1-phosphate.
Tran TH, Christoffersen S, Allan PW, Parker WB, Piskur J, Serra I, Terreni M, Ealick SE.   +7 more
europepmc   +3 more sources

Nucleoside Phosphorylases Make N7-Xanthosine, the “Non-native” Regioisomer of Xanthosine [PDF]

, 2023
Modern, highly evolved, nucleoside-processing enzymes are known to exhibit perfect regioselectivity over the glycosylation of purine nucleobases at N9. We herein report an exception to this paradigm.
Anke, Kurreck, Sarah, Westarp, Peter, Neubauer, Felix, Brandt, Sebastian, Kemper, Felix, Kaspar, Christina, Betz, Lena, Neumair, Christoph R., Jacob, Amin, Dagane   +9 more
core   +2 more sources

Synthesis of 5-oxymethyl-1,2,4-triazole-3-carboxamides

Тонкие химические технологии, 2022
Objectives. A key step in the synthesis of natural nucleoside analogs is the formation of a glycosidic bond between the carbohydrate fragment and the heterocyclic base. Glycosylation methods differ in terms of regio- and stereoselectivity.
L. E. Grebenkina, A. N. Prutkov, A. V. Matveev, M. V. Chudinov   +3 more
doaj   +1 more source