Results 21 to 30 of about 112 (103)

Thermus thermophilus Nucleoside Phosphorylases Active in the Synthesis of Nucleoside Analogues [PDF]

Applied and Environmental Microbiology, 2012
ABSTRACT Cells extracts from Thermus thermophilus HB27 express phosphorolytic activities on purines and pyrimidine nucleosides. Five putative encoding genes were cloned and expressed in Escherichia coli , and the corresponding recombinant proteins were purified and studied.
Almendros, Marcos, Berenguer, José, Sinisterra, José Vicente   +2 more
openaire   +3 more sources

A specific adenosine phosphorylase, distinct from purine nucleoside phosphorylase

FEBS Letters, 1976
Several enzymes, catalyzing the reversible phosphorolysis of purine nucleosides have been described in eucaryotic cells and in micro-organisms [l-6] . The best known is the ‘purine nucleoside phosphorylase’ (EC 2.4.2.1) acting on the nucleosides of hypoxanthine and guanine. Adenosine is not used as substrate by this enzyme [6-81.
SENESI, SONIA, FALCONE, GIUSEPPE, MURA, UMBERTO, SGARRELLA F, IPATA, PIERO LUIGI   +4 more
openaire   +5 more sources

Engineering a Bifunctional Fusion Purine/Pyrimidine Nucleoside Phosphorylase for the Production of Nucleoside Analogs

Biomolecules
Nucleoside phosphorylases (NPs) are pivotal enzymes in the salvage pathway, catalyzing the reversible phosphorolysis of nucleosides to produce nucleobases and α-D-ribose 1-phosphate.
Daniel Hormigo, Jon Del Arco, Javier Acosta, Maximilian J. L. J. Fürst, Jesús Fernández-Lucas   +4 more
doaj   +1 more source

Inosine‐Triphosphate‐Pyrophosphatase Activity as a Potential Predictor of Methotrexate Remission in Juvenile Idiopathic Arthritis

Arthritis &Rheumatology, EarlyView.
Objective Methotrexate (MTX) is the first‐line therapy for juvenile idiopathic arthritis (JIA), but up to 40% of patients do not respond to it. Low inosine triphosphate pyrophosphatase (ITPA) activity has been associated with reduced clinical remission. We investigated the role and underlying mechanisms of ITPA in vitro. Methods ITPA enzymatic activity
Sofia Sindici Forgiarini, Marianna Lucafò, Debora Curci, Davide Selvestrel, Valentina Moressa, Sofia Pagarin, Barbara Bellich, Martina Franzin, Alberto Tommasini, Mara L. Becker, Laura B. Ramsey, Susan D. Thompson, Carl D. Langefeld, Federica Corba, Edoardo Marrani, Gabriele Simonini, Gabriele Stocco, Giuliana Decorti, Andrea Taddio, Serena Pastore   +19 more
wiley   +1 more source

PNP (Purine Nucleoside Phosphorylase) [PDF]

Atlas of Genetics and Cytogenetics in Oncology and Haematology, 2018
The purine nucleoside phosphorylase gene (PNP) encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. PNP is ubiquitously expressed in mammalian cells and tissues. PNP mutations cause nucleoside phosphorylase deficiency which result in defective T cell mediated immunity but can also affect B cell immunity and antibody ...
Gurbanov, Rafig, Tunçer, Sinem
openaire   +3 more sources

Proteome Analysis of Corynebacterium diphtheriae–Macrophage Interaction

PROTEOMICS, EarlyView.
ABSTRACT Contact of Corynebacterium diphtheriae with macrophages induces adaptations on both bacterial and cellular sides. The study presented here was aiming to shed light on the simultaneous intracellular adaptation of the bacteria and changes in the proteome of the phagocytes in response to the internalization of C. diphtheriae.
Luca Musella, Jens Möller, Christopher Lischer, Julio Vera‐Gonzalez, Jörg Hofmann, Lisa Ott, Andreas Burkovski   +6 more
wiley   +1 more source

Biosynthesis of Arabinoside from Sucrose and Nucleobase via a Novel Multi-Enzymatic Cascade

Biomolecules
Arabinoside and derived nucleoside analogs, a family of nucleoside analogs, exhibit diverse typically biological activities and are widely used as antibacterial, antiviral, anti-inflammatory, antitumor, and other drugs in clinical and preclinical trials.
Yuxue Liu, Erchu Yang, Xiaojing Zhang, Xiaobei Liu, Xiaoting Tang, Zhenyu Wang, Hailei Wang   +6 more
doaj   +1 more source

MTAP Deficiency as a Metabolic Vulnerability in Cancer: Implications for Synthetic Lethal Therapy

Cancer Science, EarlyView.
MTAP deletion creates a therapeutically actionable metabolic vulnerability through MTA accumulation and PRMT5 dependency. This review summarizes the biochemical basis of MTAP‐directed synthetic lethality, emerging PRMT5/MAT2A inhibitors, clinicogenomic features of MTAP‐deleted tumors, and future strategies for precision oncology.
Hiroaki Ikushima, Hidenori Kage
wiley   +1 more source

Identification of Distinct Interacting Proteins of Giardia lamblia Centrins Using Yeast Two‐Hybrid Assays

Journal of Eukaryotic Microbiology, Volume 73, Issue 4, July/August 2026.
ABSTRACT Giardia lamblia, a protozoan parasite that causes diarrhea in humans, contains two centrins implicated in cellular morphology and cell division. To identify proteins interacting with each G. lamblia centrin (GlCent), we performed a yeast two‐hybrid assay.
Mee Young Shin, Juri Kim, Soon‐Jung Park   +2 more
wiley   +1 more source

Synthesis of Substituted 1,2,4-Triazole-3-Thione Nucleosides Using E. coli Purine Nucleoside Phosphorylase

Biomolecules
1,2,4-Triazole derivatives have a wide range of biological activities. The most well-known drug that contains 1,2,4-triazole as part of its structure is the nucleoside analogue ribavirin, an antiviral drug. Finding new nucleosides based on 1,2,4-triazole
Ilya V. Fateev, Sobirdjan A. Sasmakov, Jaloliddin M. Abdurakhmanov, Abdukhakim A. Ziyaev, Shukhrat Sh. Khasanov, Farkhod B. Eshboev, Oybek N. Ashirov, Valeriya D. Frolova, Barbara Z. Eletskaya, Olga S. Smirnova, Maria Ya. Berzina, Alexandra O. Arnautova, Yulia A. Abramchik, Maria A. Kostromina, Alexey L. Kayushin, Konstantin V. Antonov, Alexander S. Paramonov, Valeria L. Andronova, Georgiy A. Galegov, Roman S. Esipov, Shakhnoz S. Azimova, Anatoly I. Miroshnikov, Irina D. Konstantinova   +22 more
doaj   +1 more source