In vitro substrate reduction, chaperone and immunomodulation treatments reduce heparan sulfate in mucolipidosis III human fibroblasts [PDF]
Mucolipidosis II and III (MLII and MLIII) are autosomal recessive diseases caused by pathogenic variants in GNPTAB and GNPTG genes that lead to defects in GlcNAc-1-phosphotransferase. This enzyme adds mannose 6-phosphate residues to lysosomal hydrolases,
Fernanda Sperb-Ludwig +4 more
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Selection of co-transformed Dendrobium Sonia 17 using hygromycin and green fluorescent protein
Dendrobium Sonia 17 calluses were used for co-transformation study using particle bombardment. The bombarded transformed callus tissues were selected using half-strength MS medium containing 25 mg dm-3 hygromycin. Expression of green fluorescent protein (
C. S. Tee +3 more
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The widely conserved LytR-CpsA-Psr (LCP) family of enzymes in Gram-positive bacteria is known to attach glycopolymers, including wall teichoic acid, to the cell envelope.
Sara D. Siegel +6 more
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Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha. [PDF]
We previously reported Agrobacterium-mediated transformation methods for the liverwort Marchantia polymorpha using the hygromycin phosphotransferase gene as a marker for selection with hygromycin.
Kimitsune Ishizaki +7 more
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Identification and Characterization of a Novel Strain of Bacillus safensis M7L4 Capable of Degrading Zearalenone and Analysis of Related Key Enzymes [PDF]
A bacterial strain with high efficiency of zearalenone (ZEN) removal was isolated from wheat samples by enrichment culture method using ZEN as a major carbon source.
ZHAO Chengcheng, SUN Changpo, SUN Jing, WANG Jun, LIU Hujun
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Synthesis of molecular species of glycerophospholipids from diglyceride-labeled brain microsomes
Selectivity of CDP-choline:diacylglycerol choline phosphotransferase and CDP-ethanolamine:diacylglycerol ethanolamine phosphotransferase for molecular species of diglyceride has been studied in rat brain microsomes in vitro.
R Roberti, L Binaglia, G Porcellati
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Functional characterization of the phosphotransferase system in Parageobacillus thermoglucosidasius
Parageobacillus thermoglucosidasius is a thermophilic bacterium characterized by rapid growth, low nutrient requirements, and amenability to genetic manipulation. These characteristics along with its ability to ferment a broad range of carbohydrates make
Gonzalo N. Bidart +2 more
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Ethanolamine phosphotransferase (EPT) is a key enzyme responsible for the synthesis of ethanolamine glycerophospholipids. Plasmenylethanolamine is a predominant molecular subclass of ethanolamine glycerophospholipids in the heart.
David A. Ford
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Biosynthesis of phosphatidylcholine by enzyme preparations from spinach leaves
The enzymic incorporation of choline-1,2-14C from CDP-choline-1,2-14C into phosphatidylcholine by spinach leaf preparations was characterized.The enzyme catalyzing the incorporation, choline phosphotransferase, had a pH optimum of about 8.0 and required ...
K.A. DEVOR, J.R. MUDD
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Streptococcus pneumoniae is a frequent bacterial pathogen of the human respiratory tract causing pneumonia, meningitis and sepsis, a serious healthcare burden in all age groups. S.
Malgorzata Magoch +7 more
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