Results 91 to 100 of about 25,251 (292)

Differential contributions of phosphotransferases CEPT1 and CHPT1 to phosphatidylcholine homeostasis and lipid droplet biogenesis

open access: yesJournal of Biological Chemistry, 2023
G. Dorighello   +4 more
semanticscholar   +1 more source

Purification and characterization of a hygromycin B phosphotransferase from Streptomyces hygroscopicus [PDF]

open access: yes, 1987
4 pages, 4 figures, 1 table, 20 references.A hygromycin B phosphotransferase activity from Streptomyces hygroscopicus has been highly purified by ammonium sulphate fractionation followed by affinity column chromatography through Sepharose-6B- hygromycin ...
Jiménez Díaz, Antonio   +2 more
core   +1 more source

Duckweed‐based edible vaccine confers complete protection against avian infectious bronchitis virus by inducing robust mucosal and systemic immunity

open access: yesPlant Biotechnology Journal, EarlyView.
Summary Molecular farming using edible plants offers a cost‐effective strategy for vaccine production because plants serve as both bioreactors and oral delivery systems. Owing to its rapid clonal propagation and high protein content, duckweed represents a promising platform for this purpose. Here, we developed an edible duckweed‐based vaccine targeting
Xiao Tan   +12 more
wiley   +1 more source

Identification of Primary Antimicrobial Resistance Drivers in Agricultural Nontyphoidal Salmonella enterica Serovars by Using Machine Learning

open access: yesmSystems, 2019
Nontyphoidal Salmonella (NTS) is a leading global cause of bacterial foodborne morbidity and mortality. Our ability to treat severe NTS infections has been impaired by increasing antimicrobial resistance (AMR).
Finlay Maguire   +4 more
doaj   +1 more source

Glucose-1-phosphotransferase and N-acetylglucosamine-1-phosphotransferase have distinct acceptor specificities.

open access: yesJournal of Biological Chemistry, 1987
UDP-glucose:glycoprotein glucose-1-phosphotransferase (Glc-phosphotransferase) catalyzes the transfer of alpha Glc-1-P from UDP-Glc to endoglycosidase H-sensitive oligosaccharides on acceptor glycoproteins. We have previously demonstrated that Glc-phosphotransferase was specific for UDP-Glc as its nucleotide sugar substrate and thus appeared to be ...
Richard B. Marchase   +2 more
openaire   +3 more sources

Non-traditional platinum compounds for improved accumulation, oral bioavailability, and tumor targeting [PDF]

open access: yes, 2009
The five platinum anticancer compounds currently in clinical use conform to structure–activity relationships formulated (M. J. Cleare and J. D. Hoeschele, Bioinorg. Chem., 1973, 2, 187–210) shortly after the discovery that cis-diamminedichloroplatinum(II)
Altman   +86 more
core   +1 more source

Seamless, modular binary vectors for plant cell and molecular biology

open access: yesPlant Biotechnology Journal, EarlyView.
Summary In plants, genetic manipulations are commonly performed using Agrobacterium tumefaciens, a plant pathogen whose abilities as a ‘natural genetic engineer’ have been co‐opted for biotechnological applications. In the widely used binary vector systems, Agrobacterium already contains a plasmid with most of the genes needed for virulence and is ...
Rory Greenhalgh   +5 more
wiley   +1 more source

Simultaneous Knockout of Tk1‐SST and Tk1‐FFT via CRISPR/Cas9 Enhances the Natural Rubber Accumulation in Taraxacum kok‐saghyz

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT Taraxacum kok‐saghyz (TKS) synthesises natural rubber (NR) and inulin using sucrose as a carbon source. However, molecular mechanisms regulating inulin and NR accumulation remain largely unclear. Here, we report the generation of double‐gene homozygous mutants, 1‐sst1‐fft, by simultaneously knocking out two key genes responsible for inulin ...
Bi Qin   +13 more
wiley   +1 more source

Heterologous Production of Cyprosin B in Nicotiana benthamiana: Unveiling the Role of the Plant‐Specific Insert Domain in Protein Function and Subcellular Localisation

open access: yesPlant Biotechnology Journal, EarlyView.
ABSTRACT Plant systems have gained increased attention as an alternative platform for producing heterologous proteins, particularly for industrially relevant proteins. The Cynara cardunculus L. flower extract is traditionally used in cheese production across Mediterranean countries due to its milk‐clotting properties.
Saraladevi Muthusamy   +8 more
wiley   +1 more source

Identification of Benzo[a]pyrene-metabolizing bacteria in forest soils by using DNA-based stable-isotope probing [PDF]

open access: yes, 2015
DNA-based stable-isotope probing (DNA-SIP) was used in this study to investigate the uncultivated bacteria with benzo[a]pyrene (BaP) metabolism capacities in two Chinese forest soils (Mt. Maoer in Heilongjiang Province and Mt. Baicaowa in Hubei Province).
Jiang, Longfei   +5 more
core   +1 more source

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