Results 11 to 20 of about 62,445 (296)
Numerical Simulation of the Photobleaching Process in Laser-Induced Fluorescence Photobleaching Anemometer [PDF]
Micromachines, 2021 At present, a novel flow diagnostic technique for micro/nanofluidics velocity measurement—laser-induced fluorescence photobleaching anemometer (LIFPA)—has been developed and successfully applied in broad areas, e.g., electrokinetic turbulence in ...
Yu Chen +4 more
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The Impact of Photobleaching on Microarray Analysis [PDF]
Biology, 2015 DNA-Microarrays have become a potent technology for high-throughput analysis of genetic regulation. However, the wide dynamic range of signal intensities of fluorophore-based microarrays exceeds the dynamic range of a single array scan by far, thus ...
Marcel von der Haar +5 more
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Nonexponential statistics of fluorescence photobleaching [PDF]
The Journal of Chemical Physics, 2004 In this paper, I consider theoretical models of the decay via photobleaching of a sample of surface-immobilized fluorescent molecules excited by a spatially varying laser intensity profile. I show that, with mild restrictions on the photobleaching mechanism, the fluorescence decay measured in a nonuniform excitation profile is always nonexponential ...
Berglund, Andrew J., Andrew J. Berglund
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Multispectral imaging and autofluorescence photobleaching combined in a multi-head neural network for skin cancer classification [PDF]
Frontiers in MedicineIntroductionThe classification of hyperspectral images for skin cancer presents a significant challenge due to high data dimensionality and subtle differences between melanoma and non-melanoma tissues. This study investigates the efficacy of multi-target,
Vilen Jumutc +5 more
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Photobleaching in Two-Photon Excitation Microscopy [PDF]
Biophysical Journal, 2000 The intensity-squared dependence of two-photon excitation in laser scanning microscopy restricts excitation to the focal plane and leads to decreased photobleaching in thick samples. However, the high photon flux used in these experiments can potentially lead to higher-order photon interactions within the focal volume.
Patterson, George H., Piston, David W.
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Photobleaching Photoacid Generator.
Journal of Photopolymer Science and Technology, 2001 A cyclopropyl group was introduced into the photoacid generator in order to give the photobleaching property at 193nm. Cyclopropyldiphenylsulfonium trifluoromethanesulfonate (CpDP-Tf) was synthesized for this photobleaching effect. This compound is stable up to 165°C. The absorbance of CpDP-Tf at 193nm decreases upon exposure.
Kim, J.-B. +3 more
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Biophysical Journal, 1991 In the original theoretical development of fluorescence photobleaching recovery with circular or Gaussian laser intensity profiles (Axelrod et al., 1976, Biophys. J.) the bleaching process is assumed to obey first order kinetics in the fluorescent probe.
Bjarneson, D.W., Petersen, N.O.
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Sensors, 2022 The phase separation and aggregation of proteins are hallmarks of many neurodegenerative diseases. These processes can be studied in living cells using fluorescent protein constructs and quantitative live-cell imaging techniques, such as fluorescence ...
Daniel Wüstner
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Spectroscopic study of methylene blue photophysical properties in biological media
Biomedical Photonics, 2023 A spectroscopic study of the photophysical properties of methylene blue (MB) in aqueous solutions was carried out. Absorption and fluorescence spectra as well as fluorescence lifetime were recorded. The concentration dependence of the intensity and shape
D. V. Pominova +5 more
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Photo-Switching of Protein Dynamical Collectivity
Photonics, 2021 We examine changes in the picosecond structural dynamics with irreversible photobleaching of red fluorescent proteins (RFP) mCherry, mOrange2 and TagRFP-T.
Mengyang Xu +3 more
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