Results 41 to 50 of about 53,053 (260)
FEBS Open Bio, EarlyView.RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
European Journal of Histochemistry, 2019 In fluorescence microscopy, light radiation can be used to bleach fluorescent molecules in formalin-fixed and paraffin-embedded (FFPE) samples, in order to increase the ratio between signal of interest and background autofluorescence.
Claudio Pigoli +5 more
doaj +1 more source
Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae
FEBS Open Bio, EarlyView.Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj +3 more
wiley +1 more source
Optimization of Cyanine Dye Stability and Analysis of FRET Interaction on DNA Microarrays
Biology, 2016 The application of DNA microarrays for high throughput analysis of genetic regulation is often limited by the fluorophores used as markers. The implementation of multi-scan techniques is limited by the fluorophores’ susceptibility to photobleaching when ...
Marcel von der Haar +6 more
doaj +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Strongly coupled plasmon-exciton polaritons for photobleaching suppression
NanophotonicsStrong light–matter interactions have received a lot of attention, for example in the pursuit of plasmonic-excitonic structures as coherent light sources with low-power threshold.
Anulytė Justina +4 more
doaj +1 more source
Dissolved organic matter photolysis in Canadian arctic thaw ponds
Environmental Research Letters, 2013 The abundant thaw lakes and ponds in the circumarctic receive a new pool of organic carbon as permafrost peat soils degrade, which can be exposed to significant irradiance that potentially increases as climate warms and ice cover shortens.
Isabelle Laurion, Natalie Mladenov
doaj +1 more source
Efficient virus-induced gene silencing in Brassica rapa using a turnip yellow mosaic virus vector
Biologia Plantarum, 2018 Virus-induced gene silencing (VIGS) is a post-transcriptional gene silencing method used for unraveling gene functions. As an attractive alternative to mutant collections or stable transgenic plants, it has been widely used in reverse-genetics studies ...
J. Yu +9 more
doaj +1 more source
Nonexponential statistics of fluorescence photobleaching [PDF]
The Journal of Chemical Physics, 2004 In this paper, I consider theoretical models of the decay via photobleaching of a sample of surface-immobilized fluorescent molecules excited by a spatially varying laser intensity profile. I show that, with mild restrictions on the photobleaching mechanism, the fluorescence decay measured in a nonuniform excitation profile is always nonexponential ...
openaire +3 more sources