Results 61 to 70 of about 4,090 (169)

Engineering Conditional Transgene Expression in Nicotiana benthamiana

Plant Biotechnology Journal, Volume 24, Issue 1, Page 18-30, January 2026.
ABSTRACT Nicotiana benthamiana has emerged as a premier plant biofactory for recombinant protein and metabolite production due to its high metabolic versatility, ease of cultivation and permissiveness to transient expression vectors. However, challenges such as transgene silencing, low yields and metabolic toxicity limit its scalability. Synthetic gene
Elena Garcia‐Perez, Marta Vazquez‐Vilar, Diego Orzaez   +2 more
wiley   +1 more source

A Deep Metagenomic Snapshot as a Proof‐of‐Concept for Resource Generation: Simultaneous Assembly of Host, Food, and Microbiome Genomes From Stingless Bee Larval Food

Ecology and Evolution, Volume 15, Issue 12, December 2025.
This study serves as a proof‐of‐concept, demonstrating that deep shotgun sequencing of a single complex sample—stingless bee larval food—can simultaneously generate multiple genomic resources. From this dataset, we assembled three genomes (a near‐complete bacterium, a draft host mitochondrion, and a fragmented plant chloroplast) with varying ...
Carlos Ueira‐Vieira, Ana Carolina Costa Santos, Thayane Nogueira Araújo, Solange Cristina Augusto, Natanael Borges de Avila, Ana Maria Bonetti, Anderson Rodrigues dos Santos   +6 more
wiley   +1 more source

A new recombineering system for Photorhabdus and Xenorhabdus [PDF]

Nucleic Acids Research, 2014
Precise and fluent genetic manipulation is still limited to only a few prokaryotes. Ideally the highly advanced technologies available in Escherichia coli could be broadly applied. Our efforts to apply lambda Red technology, widely termed 'recombineering', in Photorhabdus and Xenorhabdus yielded only limited success.
Yin, J., Zhu, H., Xia, L., Ding, X., Hoffmann, T., Hoffmann, M., Bian, X., Müller, R., Fu, J., Stewart, A., Zhang, Y.   +10 more
openaire   +3 more sources

The Photorhabdus asymbiotica virulence cassettes deliver protein effectors directly into target eukaryotic cells

eLife, 2019
Photorhabdus is a highly effective insect pathogen and symbiont of insecticidal nematodes. To exert its potent insecticidal effects, it elaborates a myriad of toxins and small molecule effectors.
Isabella Vlisidou, Alexia Hapeshi, Joseph RJ Healey, Katie Smart, Guowei Yang, Nicholas R Waterfield   +5 more
doaj   +1 more source

Glycoproteomics and Its Role in Understanding Bacterial O‐Linked Glycosylation

PROTEOMICS, Volume 25, Issue 21-22, Page 160-176, November 2025.
ABSTRACT Protein glycosylation is now recognized as a ubiquitous process observed in all domains of life. Within bacterial species, carbohydrates can be attached to multiple residues with glycosylation of serine, threonine, or tyrosine residues via their hydroxyl side chains referred to as O‐linked glycosylation.
Kristian I. Karlic, Hamza Tahir, Nichollas E. Scott   +2 more
wiley   +1 more source

Identification of Photorhabdus symbionts by MALDI-TOF MS

Microbiology, 2020
Species of the bacterial genus Photorhabus live in a symbiotic relationship with Heterorhabditis entomopathogenic nematodes. Besides their use as biological control agents against agricultural pests, some
Virginia Hill, Peter Kuhnert, Matthias Erb, Ricardo A. R. Machado   +3 more
openaire   +2 more sources

Insecticidal Activity of Photorhabdus luminescens against Drosophila suzukii [PDF]

Insects, 2018
Drosophila suzukii causes considerable economic damage to small and thin-skinned fruits including cherry, blueberry, raspberry, grape and strawberry. Since it attacks fruits at the ripening stage, the use of chemical pesticides is limited due to the high risk of residues on fruit.
Rady Shawer, Irene Donati, Antonio Cellini, Francesco Spinelli, Nicola Mori   +4 more
openaire   +4 more sources

Photorhabdus asymbiotica — a new representative of the gram-negative microflora in urinary tract infections

RUDN Journal of Medicine, 2013
The article presents results of clinical case. New pathogen, a gram-negative bacterium Photorhabdus asymbiotica, was isolated. Identified a new causative agent of acute pyelonephritis on the background of urolithiasis — a gram-negative bacterium ...
V P Avdoshin, O V Makarov, A A Kirichek, M S Shekhtman, K S Kesh’yan   +4 more
doaj  

Report on Vibrio Species Contamination in Shrimp From the Coast of Pangandaran, West Java, Indonesia

Environmental Microbiology Reports, Volume 17, Issue 5, October 2025.
Shrimp samples from Pangandaran, Indonesia, revealed contamination by Vibrio parahaemolyticus and Vibrio alginolyticus. Toxin gene screening showed the presence of toxR but the absence of highly pathogenic markers (tdh, trh, pirA, pirB). These findings highlight infection risks and the need for improved biosecurity in shrimp aquaculture.
Titin Herawati, Indriyani Rahayu, Aisyah Aisyah, Mochamad Untung Kurnia Agung, Buntora Pasaribu, Atikah Nurhayati, Adiana Ghazali, Roffi Grandiosa, Thallita Nasywa Faddilah, Rendika Kamiswara   +9 more
wiley   +1 more source

The loss of the urea cycle and ornithine metabolism in different insect orders: An omics approach

Insect Molecular Biology, Volume 34, Issue 5, Page 632-644, October 2025.
Among urea cycle enzymes, only the nitric oxide synthase gene is universally present across insect genomes. All Hemiptera species lack the enzymes needed to convert citrulline to arginine, and some also lack the pathway from arginine to ornithine. Putrescine and spermidine synthesis is conserved in all insects, but aphids lack the capability to produce
Jessica Cristina Silva Martins, Héctor Antônio Assunção Romão, Carolina Kurotusch Canettieri, Amanda Caetano Cercilian, Patrícia Rasteiro Ordiale Oliveira, Clelia Ferreira, Walter R. Terra, Renata de Oliveira Dias   +7 more
wiley   +1 more source