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Cryo-EM structure of Chlamydomonas reinhardtii Photosystem I complexed with cytochrome c 6 [PDF]

open access: yesNature Communications
Photosynthetic electron transfer relies on small soluble carriers that shuttle electrons between the cytochrome b₆f complex and Photosystem I (PSI). While copper-containing plastocyanin (Pc) serves this role in plants, the heme protein cytochrome c₆ (Cyt
Yu Ogawa   +6 more
doaj   +2 more sources

An efficient clear-native PAGE–based workflow for cryo-electron microscopy sample preparation of large protein complexes [PDF]

open access: yesPlant Methods
Background Cryo-electron microscopy (cryo-EM) has revolutionized protein research by enabling high-resolution structural analysis. However, preparing ultra-large protein complexes (e.g., > 700 kDa) for cryo-EM remains challenging, as it requires ...
Zitong Yang   +6 more
doaj   +2 more sources

Photosynthetic Light Harvesting and Thylakoid Organization in a CRISPR/Cas9 Arabidopsis Thaliana LHCB1 Knockout Mutant

open access: yesFrontiers in Plant Science, 2022
Light absorbed by chlorophylls of Photosystems II and I drives oxygenic photosynthesis. Light-harvesting complexes increase the absorption cross-section of these photosystems.
Hamed Sattari Vayghan   +10 more
doaj   +1 more source

Structure of Photosystem I Supercomplex Isolated from a Chlamydomonas reinhardtii Cytochrome b6f Temperature-Sensitive Mutant

open access: yesBiomolecules, 2023
The unicellular green alga, Chlamydomonas reinhardtii, has been widely used as a model system to study photosynthesis. Its possibility to generate and analyze specific mutants has made it an excellent tool for mechanistic and biogenesis studies.
Tom Schwartz   +3 more
doaj   +1 more source

The Assembly of Super-Complexes in the Plant Chloroplast

open access: yesBiomolecules, 2021
Increasing evidence has revealed that the enzymes of several biological pathways assemble into larger supramolecular structures called super-complexes. Indeed, those such as association of the mitochondrial respiratory chain complexes play an essential ...
Kezhen Qin   +2 more
doaj   +1 more source

Self-assembling peptide detergents stabilize isolated photosystem I on a dry surface for an extended time. [PDF]

open access: yesPLoS Biology, 2005
We used a class of designed peptide detergents to stabilize photosystem I (PS-I) upon extended drying under N2 on a gold-coated-Ni-NTA glass surface. PS-I is a chlorophyll-containing membrane protein complex that is the primary reducer of ferredoxin and ...
Patrick Kiley   +5 more
doaj   +2 more sources

Algal photosystem I dimer and high-resolution model of PSI-plastocyanin complex

open access: yesNature Plants, 2022
Photosystem I (PSI) enables photo-electron transfer and regulates photosynthesis in the bioenergetic membranes of cyanobacteria and chloroplasts. Being a multi-subunit complex, its macromolecular organization affects the dynamics of photosynthetic ...
A. Naschberger   +10 more
semanticscholar   +1 more source

Photosynthetic Production of Molecular Oxygen by Water Oxidation

open access: yesOxygen, 2022
This review deals with the production of oxygen by photo-oxidation of water, which is a topic fitting a journal devoted to oxygen. Most of the present biosphere, including mankind, depends on oxygen. Elucidating the mechanism is of importance for solving
Lars Olof Björn
doaj   +1 more source

Formation of singlet oxygen by decomposition of protein hydroperoxide in photosystem II. [PDF]

open access: yesPLoS ONE, 2017
Singlet oxygen (1O2) is formed by triplet-triplet energy transfer from triplet chlorophyll to O2 via Type II photosensitization reaction in photosystem II (PSII). Formation of triplet chlorophyll is associated with the change in spin state of the excited
Vinay Pathak   +2 more
doaj   +1 more source

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