Results 141 to 150 of about 13,180,176 (302)

The NT hunting for health challenge lesson pack

open access: yes, 2005
The Committee for the NT Hunting for Health Challenge gratefully acknowledges the support of the Pilbara Public Health Unit in permitting the adaptation of their resource for use in the Northern Territory.The NT hunting for health challenge lesson pack ...
Nutrition and Physical Activity Program
core   +2 more sources

Exercise‐induced changes in left ventricular strain are affected by interleukin‐6 activity: An exploratory analysis of a randomised‐controlled trial in humans with abdominal obesity

open access: yesExperimental Physiology
Whilst the exercise‐induced myokine interleukin‐6 (IL‐6) plays a beneficial role in cardiac structural adaptations, its influence on exercise‐induced functional cardiac outcomes remains unknown. We hypothesised that IL‐6 activity is required for exercise‐
Simon Jønck   +12 more
doaj   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

open access: yesFEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj   +3 more
wiley   +1 more source

Post-disaster context influencing Quality of Life (QoL) of adolescents: the role of organized physical activity

open access: yes, 2018
Environmental determinants of QoL are dramatic in a post-disaster context, particularly in children. In 2015, a cross-sectional survey has been carried out on a sample of 722 adolescents (50.0% males, 13.6 years average age) from two towns in Central ...
Fabiani L.   +5 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

Exploring sedentary behaviour reduction interventions in the primary healthcare setting: a scoping review

open access: yesBMC Primary Care
Background Excessive sitting time is a risk factor for many non-communicable diseases and an increased risk of early mortality. Primary healthcare is a viable setting to target population health behaviour change.
Aaron Beecroft   +4 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

Cluster analysis of physical activity and physical fitness and their associations with components of school skills in children aged 8–9 years

open access: yesScientific Reports
This study aimed to cluster physical activity (PA), physical fitness, visual-motor coordination, household duties, and their associations with school skills in 170 primary school children (88 girls and 82 boys) aged 8–9 years.
Agata Korcz   +7 more
doaj   +1 more source

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