Results 71 to 80 of about 116,652 (327)

Using a Supramolecular Approach to Engineer Modular Hydrogel Platforms for Culturing Protoplasts – from General Tissue Engineering to Cellular Agriculture

Advanced Biology, EarlyView.
Using supramolecular monomers, various hydrogel culture systems were formulated to culture protoplasts; including 2D, 2.5D, and 3D hydrogels. Depending on the culture platform, bioactive functionalization led to protoplast enlargement (2D and 2.5D) or plasmolysis (3D). This work shows the potential to modularly engineer synthetic platforms for cellular
Maritza M. Rovers, Erik J. Slootweg, Ferdinand C. O. Los, Patricia Y. W. Dankers   +3 more
wiley   +1 more source

Local unfolding of the HSP27 monomer regulates chaperone activity

Nature Communications, 2019
The small heat-shock protein HSP27 occurs predominantly in oligomeric forms, which makes its structural characterisation challenging. Here the authors employ CPMG and high-pressure NMR with native mass spectrometry and biophysical assays to show that the
T. Reid Alderson, Julien Roche, Heidi Y. Gastall, David M. Dias, Iva Pritišanac, Jinfa Ying, Ad Bax, Justin L. P. Benesch, Andrew J. Baldwin   +8 more
doaj   +1 more source

Site Selective and Efficient Spin Labeling of Proteins with a Maleimide-Functionalized Trityl Radical for Pulsed Dipolar EPR Spectroscopy

Molecules, 2019
Pulsed dipolar electron paramagnetic resonance spectroscopy (PDS) in combination with site-directed spin labeling (SDSL) of proteins and oligonucleotides is a powerful tool in structural biology.
J. Jacques Jassoy, Caspar A. Heubach, Tobias Hett, Frédéric Bernhard, Florian R. Haege, Gregor Hagelueken, Olav Schiemann   +6 more
doaj   +1 more source

Hydrogel‐Based Capacitive Sensor Model for Ammonium Monitoring in Aquaculture

Advanced Engineering Materials, EarlyView.
Traditional techniques for monitoring aquaculture water quality, particularly ammonium levels, harm fish. This work presents a novel capacitive sensor with an ionic hydrogel transducer to monitor ammonium concentration in real time based on the ammonium‐induced hydrogel dissociation and osmotic pressure. Monitoring aquaculture water quality, especially
Mohammad Mirzaee, Mahdi Askari‐sedeh, Ali Zolfagharian, Mostafa Baghani   +3 more
wiley   +1 more source

Bridging Nature and Technology: A Perspective on Role of Machine Learning in Bioinspired Ceramics

Advanced Engineering Materials, EarlyView.
Machine learning (ML) is revolutionizing the development of bioinspired ceramics. This article investigates how ML can be used to design new ceramic materials with exceptional performance, inspired by the structures found in nature. The research highlights how ML can predict material properties, optimize designs, and create advanced models to unlock a ...
Hamidreza Yazdani Sarvestani, Aditi Singh, Behnam Ashrafi   +2 more
wiley   +1 more source

Ultrafast carbon monoxide photolysis and heme spin-crossover in myoglobin via nonadiabatic quantum dynamics

Nature Communications, 2018
Myoglobin bound to carbon monoxide undergoes an ultrafast light-induced reaction, which ends up in a photolyzed carbon monoxide and a spin transition of the iron center.
Konstantin Falahati, Hiroyuki Tamura, Irene Burghardt, Miquel Huix-Rotllant   +3 more
doaj   +1 more source

LILBID laser dissociation curves: a mass spectrometry-based method for the quantitative assessment of dsDNA binding affinities

Scientific Reports, 2020
One current goal in native mass spectrometry is the assignment of binding affinities to noncovalent complexes. Here we introduce a novel implementation of the existing laser-induced liquid bead ion desorption (LILBID) mass spectrometry method: this new ...
Phoebe Young, Genia Hense, Carina Immer, Jens Wöhnert, Nina Morgner   +4 more
doaj   +1 more source

Theoretical constraints of physical and chemical properties of hydrothermal fluids on variations in chemolithotrophic microbial communities in seafloor hydrothermal systems

Progress in Earth and Planetary Science, 2014
In the past few decades, chemosynthetic ecosystems at deep-sea hydrothermal vents have received attention as plausible analogues to the early ecosystems of Earth, as well as to extraterrestrial ecosystems.
Kentaro Nakamura, K. Takai
semanticscholar   +1 more source

A Different Perspective on the Solid Lubrication Performance of Black Phosphorous: Friend or Foe?

Advanced Engineering Materials, EarlyView.
Researchers investigate black phosphorous (BP) as a standalone solid lubricant coating through ball‐on‐disc linear‐reciprocating sliding experiments in dry conditions. Testing on different metals shows BP doesn't universally reduce friction and wear. However, it achieves 33% friction reduction on rougher iron surfaces and 23% wear reduction on aluminum.
Matteo Vezzelli, Manel Rodríguez Ripoll, Sabine Schwarz, Ali Erdemir, Maria Clelia Righi, Carsten Gachot   +5 more
wiley   +1 more source

Photoluminescence emission and Raman enhancement in TERS: an experimental and analytic revisiting

Nanophotonics
An analytic model is used to calculate the Raman and fluorescence enhancement of a molecule in between two closely spaced gold nanospheres. Instead of using the conventional approach that only the dipolar plasmonic mode is considered, we calculate the ...
Chen Yu-Ting, Liu Quan, Schneider Felix, Brecht Marc, Meixner Alfred J., Zhang Dai   +5 more
doaj   +1 more source