Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas +6 more
wiley +1 more source
Mapping the physical condition, peer and parental support, and MVPA: a network analysis in schoolchildren from the ACTIBESE project. [PDF]
Rodríguez-Rodríguez F +3 more
europepmc +1 more source
Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow +12 more
wiley +1 more source
Effect of Dual-Task Training on Cognitive Function and Physical Condition in Adults With Intellectual Disabilities. [PDF]
Gutiérrez-Cruz C +4 more
europepmc +1 more source
Virgin Polystyrene Microparticles Exposure Leads to Changes in Gills DNA and Physical Condition in the Mediterranean Mussel Mytilus Galloprovincialis. [PDF]
Masiá P, Ardura A, García-Vázquez E.
europepmc +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Cracks in the Foundation: The Association of Physical Condition of School Facilities With Absenteeism and Test Scores in Maryland. [PDF]
Gong CH +4 more
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Protective Factors Against Social Exclusion in Adolescents: Physical Condition and Physical Activity. [PDF]
Rodríguez-Negro J +4 more
europepmc +1 more source
Effect of the COVID-19 lockdown period on the physical condition, living habits, and physical activity of citizens in Beijing, China. [PDF]
Zhu Y +6 more
europepmc +1 more source

