Results 71 to 80 of about 513,704 (220)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Association between Physical Activity and Autonomic Nervous System in Young Sedentary Adults: A Cross-sectional Study

Journal of Clinical and Diagnostic Research
Introduction: A sedentary lifestyle is a key driver of early onset Non Communicable Diseases (NCDs). The Autonomic Nervous System (ANS), which governs involuntary functions and maintains internal balance, may be disrupted by physical inactivity in young ...
Yogeshwari Raman, Venkatesh Natarajan, Priscilla Johnson, Savita Ravindra   +3 more
doaj   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

The effect of exercise with bicycle’s inner tube on improvement of physical fitness and balance in women with metabolic syndrome

Journal of Associated Medical Sciences, 2017
Background: Metabolic syndrome is a health problem that lead to various abnormal functions of body system, including physical fitness and balance.
Kanokthip Sawangjaithum, Benjamaporn Hancharoenkul, Oranich Vimolratana, Sarayoot Mongkol   +3 more
doaj  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Comparison of physical function recovery in patients with rheumatoid arthritis and those with osteoarthritis after total knee arthroplasty

Journal of Joint Surgery and Research
Purpose: Patients with rheumatoid arthritis (RA) who have undergone primary total knee arthroplasty (TKA) have a different recovery process of walking ability compared with patients with osteoarthritis (OA).
Eriko Mashimoto, Manabu Nankaku, Gakuto Kitamura, Kohei Nishitani, Shinichiro Nakamura, Shinichi Kuriyama, Ryosuke Ikeguchi, Shuichi Matsuda   +7 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source

Virtual acoustics in inhomogeneous media with single-sided access

Scientific Reports, 2018
A virtual acoustic source inside a medium can be created by emitting a time-reversed point-source response from the enclosing boundary into the medium. However, in many practical situations the medium can be accessed from one side only.
Kees Wapenaar, Joeri Brackenhoff, Jan Thorbecke, Joost van der Neut, Evert Slob, Eric Verschuur   +5 more
doaj   +1 more source