Results 151 to 160 of about 13,429,743 (301)

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

open access: yesFEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas   +6 more
wiley   +1 more source

Quality and Patient Safety Division Annual Report 2014

open access: yes, 2015
A work plan is developed on an annual basis to support the quality and patient safety objectives in the National Service Plan. This report reviews the work completed in 2014 in delivery of the 2013 plan.
Quality Improvement Division
core  

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

open access: yesFEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow   +12 more
wiley   +1 more source

Association between Vision Impairment and Physical Quality of Life Assessed Using National Surveillance Data. [PDF]

open access: yesOptom Vis Sci, 2021
VanNasdale DA   +5 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

PHYSICAL DEVELOPMENT AND QUALITY OF LIFE IN STUDENTS

open access: yesTRAKIA JOURNAL OF SCIENCES
Physical health refers to the well-being of the physical body and is defined as the optimal functioning of its constituent parts (cells, tissues, organs and systems) and harmonious interaction between them, contributing to normal structural-functional characteristics and adaptability of the organism, good physical performance and physical development ...
openaire   +3 more sources

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

The Role of Water Quality Perceptions in Modeling Lake Recreation Demand [PDF]

open access: yes
Recreation demand models typically incorporate measures of the physical attributes of recreational sites; e.g., Secchi depth or phosphorous levels in case of water quality.
Kling, Catherine L.   +3 more
core  

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