We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Salivary Cortisol Concentration Is an Objective Measure of the Physiological Response to Loud Music
Purpose: This study examines the potential associations between salivary cortisol concentrations and subjective stress test scores in healthy individuals subjected to sound-related, psychological, and physical stressors.
Robert Tomljenović +5 more
doaj +1 more source
Gender Variations of Physiological and Psychological Stress among Police Officers [PDF]
This paper analyses the effect of gender on reported and perceived levels of stress through examination of both the physical and psychological indicators.
Martin Gächter +2 more
core
GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain +11 more
wiley +1 more source
An Analysis of Menstrual Symptoms, Menstrual Attitudes, Physical Stress and Psychological Stress According to the Menstrual Cycle Phase. [PDF]
Weon HW, Son HK.
europepmc +1 more source
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source
Assessment of physical stress during the perioperative period of endoscopic submucosal dissection. [PDF]
Chinda D, Shimoyama T.
europepmc +1 more source
The two catalytic subunits of typhoid toxin dissociate from the holotoxin in the ER of an intoxicated cell, but only CdtB exits the ER to generate immunosuppressive effects. PltA is retained in the ER and sequestered from its cytosolic target, thus allowing the anti‐inflammatory effects of CdtB to promote intestinal colonization.
Maria C. Zabala‐Rodriguez +4 more
wiley +1 more source
Effects of resilience training on mental, emotional, and physical stress outcomes in military officer cadets. [PDF]
Zueger R +4 more
europepmc +1 more source
Individual differences and occupational stress perceived: a Croatian survey [PDF]
Apart from elaborating the concept of occupational stress (through it’s definition, sources, consequences, ways of dealing with it, and it’s relationship with individual differences), the research had two objectives: (1) to measure occupational stress ...
Nina Pološki Vokić, Ana Bogdanić
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