Results 131 to 140 of about 22,994,343 (308)

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Evaluación ergonómica en una empresa del sector alimenticio venezolano

Ingenieria Industrial, 2010
Se presenta una investigación descriptiva de corte transversal, que tuvo como propósito realizar una evaluación ergonómica de carácter integral en dos áreas de una empresa de alimentos congelados (Vegetales Congelados y Bocados de Yuca), con el fin de ...
Carlos García Rondón, Eliana Rodríguez Marquez   +1 more
doaj  

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

The Influence of the Non-Physical Work Environment and Work Satisfaction on the Work Enthusiasm of Civil Servants of the Population and Civil Registration Office of Pontianak

Indonesian Interdisciplinary Journal of Sharia Economics
The effectiveness of civil servants in achieving organizational goals is greatly influenced by various factors, including work environment and job satisfaction.
Awan Kusuma, Eru Ahmadia
doaj  

Validity of self-reported exposures to work postures and manual materials handling. Stockholm MUSIC I Study Group.

, 1993
Exposure data from self-administered questionnaires on manual materials handling and work postures were validated in relation to direct measurements and systematic observations on 39 men and 58 women representing 45 different occupations.
L Karlqvist, Wiktorin, Christina,, Karlqvist, Lena, C Wiktorin, Winkel, Jörgen,, Jørgen Winkel   +5 more
core   +1 more source

Salmonella enterica serovar typhi limits the potency of typhoid toxin and ADP‐ribosylating toxin AB to establish a persistent infection

FEBS Open Bio, EarlyView.
The two catalytic subunits of typhoid toxin dissociate from the holotoxin in the ER of an intoxicated cell, but only CdtB exits the ER to generate immunosuppressive effects. PltA is retained in the ER and sequestered from its cytosolic target, thus allowing the anti‐inflammatory effects of CdtB to promote intestinal colonization.
Maria C. Zabala‐Rodriguez, Juliette K. Tinker, Teresa Frisan, Taj Azarian, Ken Teter   +4 more
wiley   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source

PENGARUH LINGKUNGAN KERJA FISIK, FASILITAS KERJA DANKOMPETENSI PEGAWAI TERHADAP SISTEM PENGELOLAAN ARSIP DIKANTOR KELURAHAN SE-KECAMATAN CILACAP UTARA

Economic Education Analysis Journal, 2017
Tujuan dari penelitian ini ialah untuk mengetahui ada tidaknya pengaruh secara simultan maupun parsial antara lingkungan kerja fisik, fasilitas kerja dan kompetensi pegawai terhadap sistem pengelolaan arsip di Kantor Kelurahan se-Kecamatan Cilacap Utara.
Shinta Salgiarti, Nanik Suryani
doaj  

Physical activity in the work environment

, 2021
Globally, one in four adults do not meet the recommended amount of physical activity. Because people spend a significant amount of time in the work environment and especially office employees spend a lot of time in sedentary behaviour and have low ...
de Graaf, Jetske (author)
core