Results 31 to 40 of about 773,674 (244)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Anchorage‐independent and faster growth in clonal population from UV‐irradiated NER‐deficient cells

FEBS Open Bio, EarlyView.
UV‐irradiated cells expressing a DDB2 mutant protein unable to interact with PCNA (DDB2PCNA‐) form clones able to grow without anchorage. Different experimental approaches reveal heterogeneity in cell cycle regulation and drug response within these clones, emphasizing the crucial role of the DDB2‐PCNA interaction in preventing cellular transformation ...
Paola Perucca, Anna Tricarico, Martina Furfaro, Priyam Ghosh, Ennio Prosperi, Lucia Anna Stivala, Ornella Cazzalini   +6 more
wiley   +1 more source

Relativistic Kramers-Pasternack Recurrence Relations

, 2009
Recently we have evaluated the matrix elements $$,$ where $O$ $={1,\beta, i\mathbf{\alpha n}\beta} $ are the standard Dirac matrix operators and the angular brackets denote the quantum-mechanical average for the relativistic Coulomb problem, in terms of ...
Andrae D, Askey R A, Atakishyev N M, Bailey W N, Baz A I, Bethe H A, Blanchard P, Cordero-Soto R Suslov S K, Ding Yi-B, Erdélyi A, Gumberidze A, Karlin S, Karshenboim S G, Karshenboim S G, Koekoek R Swarttouw R F, Kramers H A, Kramers H A, Landau L D, Liboff R L, Messiah A, More R M, Moreno B, Nikiforov A F, Nikiforov A F, Núñez-Yépez H N, Ojha P C, Pasternack S, Perelomov A M, Qiang W-C, Sergei K Suslov, Shabaev V M, Shabaev V M, Shabaev V M, Shabaev V M, Shabaev V M, Suslov S K, Swainson R A, Tchebychef P L, Tchebychef P L, Tchebychef P L, Tchebychef P L, Tchebychef P L, Tchebychef P L   +42 more
core   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Physical Limits on Atomic Resolution

Microscopy and Microanalysis, 2004
It is shown that the ultimate resolution is not limited by the bandwidth of the microscope but by the bandwidth (i.e., the scattering power) of the object. In the case of a crystal oriented along a zone axis, the scattering is enhanced by the channeling of the electrons.
Van Dyck, D. (author), Van Aert, S. (author), Den Dekker, A.J. (author)   +2 more
openaire   +5 more sources

Modern topics in theoretical nuclear physics

, 2006
Over the past five years there have been profound advances in nuclear physics based on effective field theory and the renormalization group. In this brief, we summarize these advances and discuss how they impact our understanding of nuclear systems and ...
A Schwenk, B K Jennings, Beane M., Bedaque U., Bender P.-H., Bertulani H.W., Bogner A., Bogner T.T.S., Bogner T.T.S., Bogner T.T.S., Braaten H.-W., Butler J.-W., Caurier G., Chin M., Cooper B.K., Entem, Epelbaum A., Epelbaum U.-G., Epelbaum W., Finelli N., Forssen P., Goriely M., Greiner C.A., Gupta Z., Halboth W., Haxton T., Horowitz A., Horowitz M.A., Jennings N., Lee K., Murthy S., Navratil J.P., Navratil W.E., Nogga H., Nogga S.K., O'Hara S.L., Pieper R.B., Pieper R.B., Roth H., Salmhofer C., Sarazin J.S., Schwenk C., Schwenk G.E., Serot J.D., Stoitsov J., Watanabe K., Wloch D.J., Zanchi H., Zhu C.M., Zwierlein A.   +49 more
core   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

Atomic physics and modern solar spectro-polarimetry

, 2017
Observational solar physics is entering a new era with the advent of new 1.5 m class telescopes with adaptive optics, as well as the Daniel K. Inouye 4 m telescope which will become operational in 2019.
Judge, Philip
core   +1 more source

Meson and Quark Degrees of Freedom and the Radius of the Deuteron [PDF]

, 1996
The existing experimental data for the deuteron charge radius are discussed. The data of elastic electron scattering are inconsistent with the value obtained in a recent atomic physics experiment.
Buchmann, A. J., Henning, H., Sauer, P. U.   +2 more
core   +2 more sources