Results 91 to 100 of about 1,258,930 (298)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Physics Nobel laureate Wolfgang Ketterle : a scientometric portrait [PDF]

, 2004
Wolfgang Ketterle was honoured with the Nobel Prize in Physics (2001) at 44 years of biological age and at 20 years of research publishing career. He had 115 publications during 1982 – 2002 in domains: Bose-Einstein Condensation (68), Laser Spectroscopy
Koganuramath, M. M., Kademani, B. S., Jange, Suresh, Kalyane, V. L., Angadi, Mallikarjun   +4 more
core  

Repetition and pair-interaction of string-like hopping motions in glassy polymers [PDF]

, 2017
2016-2017 > Academic research: refereed > Publication in refereed journalbcrcVersion of RecordPublishedVoR ...
Lam, CH, Chi-Hang Lam
core   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Characterization of the Excited State on Methanol/TiO2(110) Interface [PDF]

, 2015
The electronic structure of methanol/TiO2(110) interface has been studied by photoeraission spectroscopy. The pronounced resonance which appears at 5.5 eV above the Fermi level in two-photon photoemission spectroscopy (2PPE) is associated with the ...
Yang, Xue-ming, Hao, Qun-qing, Dong-xu Dai, Ren, Ze-feng, Dai, Dong-xu, Chuan-yao Zhou, Zhi-bo Ma, Zhou, Chuan-yao, Ze-feng Ren, Wang, Zhi-qiang, Ma, Zhi-bo, Xin-chun Mao, Zhi-qiang Wang, Xue-ming Yang, Qun-qing Hao, Mao, Xin-chun   +15 more
core   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

The dynamics of copper intercalated molybdenum ditelluride [PDF]

, 2016
2016-2017 > Academic research: refereed > Publication in refereed journal201804_a bcmaVersion of RecordPublishedVoR ...
Guzman, D, Strachan, A, David Guzman, Onofrio, N, Alejandro Strachan, Nicolas Onofrio   +5 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source