Results 61 to 70 of about 12,257,680 (242)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Pendulums and Nonlinear Effects - Warning 4000 level physics [video]

open access: yes, 2015
NPS PhysicsIn-class Physics DemonstrationsDr.
Naval Postgraduate School Physics
core   +2 more sources

Physical time and physical space in general relativity [PDF]

open access: yesAmerican Journal of Physics, 2004
This paper comments on the physical meaning of the line element in general relativity. We emphasize that, generally speaking, physical spatial and temporal coordinates (those with direct metrical significance) exist only in the immediate neighborhood of a given observer, and that the physical coordinates in different reference frames are related by ...
openaire   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

Teaching quantum interpretations : revisiting the goals and practices of introductory quantum physics courses [PDF]

open access: yes, 2015
This work was supported in part by NSF CAREER Grant No. 0448176, NSF DUE No. 1322734, NSF IUSE No. 1432204, the University of Colorado, and the University of St Andrews.Most introductory quantum physics instructors would agree that transitioning students
Charles Baily   +3 more
core   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

open access: yesFEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose   +9 more
wiley   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

open access: yesFEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

Generalized symmetry in crystal physics

open access: yesComputers & Mathematics with Applications, 1988
AbstractThe external and internal symmetry groups of the matter object and physical processes with a complex structure and the internal degrees of the freedom are combined into single groups of their generalized coloured symmetry by means of the wreath product of these groups.
openaire   +1 more source

Summary of 11th international symposium on high energy spin physics [PDF]

open access: yes, 1995
The topics discussed during the 11th Internation Symposium on High Energy Spin Physics are summarized. These topics are relevant to both High Energy and Nuclear Physics.
Krisch, A. D.   +2 more
core   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

open access: yesFEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas   +6 more
wiley   +1 more source

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