Results 41 to 50 of about 6,062,383 (272)
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
FEBS Open Bio, EarlyView.BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
FEBS Open Bio, EarlyView.Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata +3 more
wiley +1 more source
Biomimetics, 2021 In recent years, demographic growth has caused cities to expand their urban areas, increasing the risk of overheating, creating insurmountable microclimatic conditions within the urban area, which is why studies have been carried out on the urban heat ...
Kevin Araque +3 more
doaj +1 more source
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Objective This study aimed to investigate the immunological response to influenza vaccination, the incidence and severity of influenza infection, and the side effects of the vaccination in patients with ischemic stroke. Methods This prospective observational study was conducted between 2023 and 2024 at Ramathibodi Hospital.
Achiraya Pakngao +5 more
wiley +1 more source
Thermal stress risk assessment using Physiological Equivalent Temperature (PET) in Iran [PDF]
Salāmat-i kār-i ĪrānBackground and Aim Climate change and rising temperatures pose significant challenges to public health and the environment. Weather conditions critically influence human health and physiological performance.
Mehdi Asghari +6 more
doaj
Lifestyle Journal, 2022 Green infrastructure is presented in several research as an urban strategy necessary to minimize the negative effects arising from the urbanization process and provide outdoor thermal comfort.
Amanda Ramos Goulart +2 more
doaj +1 more source
A comprehensive analysis of physiologically equivalent temperature changes of Iranian selected stations for the last half century [PDF]
Theoretical and Applied Climatology, 2016 As a preliminary and major step for land use planning of the coming years, the study of variability of the past decades’ climatic conditions with comprehensive indicators is of high importance. Given the fact that one of the affected areas by climatic change includes variability of thermal comfort, this study uses the physiologically equivalent ...
Roshan Gholamreza +3 more
openaire +2 more sources
Adaptive Foam 3D Printing of Ultralight and Multifunctional Materials
Advanced Engineering Materials, EarlyView.Adaptive foam 3D printing, enabled by expandable microspheres, imparts cellular structures to thermoplastic and thermosetting polymers, manufactured through a variety of processes including fused filament fabrication, direct ink writing, digital light processing, and inkjet printing.
Nariman Rajabifar, Amir Ameli
wiley +1 more source