Results 221 to 230 of about 44,813 (273)

Heterologous expression of chimera synthetic LTB/GnRH in Pichia pastoris and its effect on immune responseand in the seminiferous epithelium of mice

, 2011
Juliano Lacava Pereira
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DNA Sequence Changes Resulting from Codon Optimization Affect Gene Expression in <i>Pichia pastoris</i> by Altering Chromatin Accessibility. [PDF]

J Fungi (Basel)
Lu C, Guo L, Fang B, Shi J, Zhou M.
europepmc   +1 more source

Evaluation of the immune effect of foot-and-mouth disease virus-like particles derived from <i>Pichia Pastoris</i> on mice and pigs. [PDF]

Front Microbiol
Li Z, Bai M, Yin S, Yang Y, Dong H, Teng Z, Sun S, Bao E, Guo H.   +8 more
europepmc   +1 more source

Structural Homology Fails to Predict Secretion Efficiency in <i>Pichia pastoris</i>: Divergent Responses of Architecturally Similar scFvs to Multi-Parametric Genetic Engineering. [PDF]

Int J Mol Sci
Wang N, Xiao Y, Liu X, Li Y, Yu D, Guo J, Lu P, Zhang X.   +7 more
europepmc   +1 more source

Metabolic engineering of Pichia pastoris

Metabolic Engineering, 2018
Besides its use for efficient production of recombinant proteins the methylotrophic yeast Pichia pastoris (syn. Komagataella spp.) has been increasingly employed as a platform to produce metabolites of varying origin. We summarize here the impressive methodological developments of the last years to model and analyze the metabolism of P.
Brigitte Gasser, Jürgen Zanghellini, Matthias G Steiger   +2 more
exaly   +3 more sources

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Dynamic Feeding for Pichia pastoris

2022
The knowledge of certain strain-specific parameters of recombinant Pichia pastoris strains is required to be able to set up a feeding regime for fed-batch cultivations. These parameters are commonly determined either by time-consuming and labor-intensive continuous cultivations or by several, consecutive fed-batch cultivations. Here, we describe a fast
Alexander, Pekarsky, Oliver, Spadiut
openaire   +2 more sources

Pichia pastoris Promoters

2019
The methylotrophic yeast Pichia pastoris (Komagataella phaffii) is used as an expression system for recombinant protein production for a variety of applications. It grows rapidly on inexpensive media containing methanol, glucose, glycerol, or ethanol as a sole carbon source. P.
Aysun, Türkanoğlu Özçelik, Semiramis, Yılmaz, Mehmet, Inan   +2 more
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Expression of proteins in Pichia pastoris

2021
The methylotrophic yeast Pichia pastoris is currently one of the most versatile and popular hosts for the production of heterologous proteins, including industrial enzymes. The popularity of P. pastoris stems from its ability to grow to high cell densities, producing high titers of secreted heterologous protein with very low amounts of endogenous ...
Giuliana, Mastropietro, Rochelle, Aw, Karen M, Polizzi   +2 more
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Optimising Pichia pastoris Induction

2012
A common method for inducing the production of recombinant proteins in Pichia pastoris is through the use of methanol. However, the by-products of methanol metabolism are toxic to yeast cells and therefore its addition to recombinant cultures must be controlled and monitored throughout the process in order to maximise recombinant protein yields ...
Zharain, Bawa, Richard A J, Darby
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Recombinant protein expression in Pichia pastoris

Molecular Biotechnology, 2000
The methylotrophic yeast Pichia pastoris is now one of the standard tools used in molecular biology for the generation of recombinant protein. P. pastoris has demonstrated its most powerful success as a large-scale (fermentation) recombinant protein production tool.
J M, Cregg, J L, Cereghino, J, Shi, D R, Higgins   +3 more
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