Results 251 to 260 of about 45,758 (267)
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De novo biosynthesis of 2′-fucosyllactose in engineered Pichia pastoris
Biotechnology Letters, 2023D. Qian +5 more
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Gene expression in yeast: Pichia pastoris
Current Opinion in Biotechnology, 1991Recent studies have shown the versatility and utility of the Pichia pastoris expression system. Improvements in strains have boosted the yield of proteins and peptides to the commercially feasible range. The Pichia pastoris expression system will soon be used to manufacture proteins for human clinical trials.
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Towards systems metabolic engineering in Pichia pastoris
Biotechnology Advances, 2017The methylotrophic yeast Pichia pastoris is firmly established as a host for the production of recombinant proteins, frequently outperforming other heterologous hosts. Already, a sizeable amount of systems biology knowledge has been acquired for this non-conventional yeast.
Jan-Philipp Schwarzhans +4 more
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Biotechnology Journal, 2019
With nearly three decades of development, Pichia pastoris (P. pastoris) has become a powerful eukaryotic protein expression system for the expression of thousands of proteins both on a laboratory and industrial scale.
T. Zhu, Hongbing Sun, Meiyu Wang, Yin Li
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With nearly three decades of development, Pichia pastoris (P. pastoris) has become a powerful eukaryotic protein expression system for the expression of thousands of proteins both on a laboratory and industrial scale.
T. Zhu, Hongbing Sun, Meiyu Wang, Yin Li
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Pathway engineering facilitates efficient protein expression in Pichia pastoris
Applied Microbiology and Biotechnology, 2022Chao Liu +7 more
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Glycosylation of Pichia pastoris ‐derived proteins
Biotechnology and Applied Biochemistry, 1999The Pichia pastoris system for expression of heterologous recombinant proteins is being used increasingly because of the large yields of properly folded proteins that result and the ease of scaling preparations into large‐biomass fermentors. Another advantage of this system centres on the type of glycosylation that results, generally yielding protein ...
Francis J. Castellino +1 more
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Expression of plant proteins in Pichia pastoris
2004Various plant proteins are to be expressed in Pichia pastoris in our ongoing project. The Invitrogen¡¦s expression vectors and strains are applied on the heterologous production and we have converted the expression vectors Gateway compatible in order to facilitate the cloning steps.
Toikkanen, Jaana H. +5 more
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A versatile toolbox for CRISPR-based genome engineering in Pichia pastoris
Applied Microbiology and Biotechnology, 2021Xihao Liao +4 more
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