Results 261 to 267 of about 45,758 (267)

Heterologous Production of Fungal Effectors in Pichia pastoris

2011
In this chapter a method for the heterologous production of fungal proteins in the yeast Pichia pastoris is described. Starting with cloning of the sequence encoding the gene of interest into the expression vector, this protocol describes P. pastoris transformation, production of the protein in a fermentor, and purification of the protein.
openaire   +4 more sources

Expression of Antibody Fragments in Pichia pastoris [PDF]

, 2003
openaire   +2 more sources

High-level constitutive expression of leech hyaluronidase with combined strategies in recombinant Pichia pastoris

Applied Microbiology and Biotechnology, 2020
Hao Huang, Qixing Liang, Yang Wang, Jian Chen, Zhen Kang   +4 more
semanticscholar   +1 more source

CRISPR/Cas9-Mediated Homology-Directed Genome Editing in Pichia pastoris.

Methods in molecular biology, 2019
T. Gassler, Lina Heistinger, D. Mattanovich, Brigitte Gasser, R. Prielhofer   +4 more
semanticscholar   +1 more source

Toward the construction of a technology platform for chemicals production from methanol: d-lactic acid production from methanol by an engineered yeast Pichia pastoris

World Journal of Microbiology & Biotechnology, 2019
Ryosuke Yamada, K. Ogura, Yusuke Kimoto, H. Ogino   +3 more
semanticscholar   +1 more source

Methanol independent induction in Pichia pastoris by simple derepressed overexpression of single transcription factors

Biotechnology and Bioengineering, 2018
T. Vogl, Lukas Sturmberger, P. Fauland, P. Hyden, J. E. Fischer, Christian Schmid, G. Thallinger, M. Geier, A. Glieder   +8 more
semanticscholar   +1 more source

Development of a general defined medium for Pichia pastoris

Biotechnology and Bioengineering, 2018
Catherine B Matthews, Angel Kuo, K. Love, J. C. Love   +3 more
semanticscholar   +1 more source