Results 111 to 120 of about 3,932,093 (328)

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

A metamorphic robot suitable for rigid miniature pipelines

Jixie chuandong
ObjectiveTo address the limited adaptability of traditional pipeline robots in complex working environments, a design proposal for a shape memory alloy (SMA) driven peristaltic metamorphic robot suitable for rigid miniature pipelines was presented ...
JIA Xiaoli, DAI Jingting, MI Baichuan, DAN Jiale, KE Liaoliang   +4 more
doaj   +2 more sources

Off the Beaten Path: Let's Replace Term-Based Retrieval with k-NN Search

, 2016
Retrieval pipelines commonly rely on a term-based search to obtain candidate records, which are subsequently re-ranked. Some candidates are missed by this approach, e.g., due to a vocabulary mismatch.
Andoni A., Beyer K., Broder A. Z., Brown P. F., Fried D., Le Q., Mikolov T., Mu Y., Muja M., Petrović S., Riezler S., Salton G., Wang J., Weber R., Yang L., Yao X.   +15 more
core   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

A comprehensive proteogenomic pipeline for neoantigen discovery to advance personalized cancer immunotherapy

Nature Biotechnology
The accurate identification and prioritization of antigenic peptides is crucial for the development of personalized cancer immunotherapies. Publicly available pipelines to predict clinical neoantigens do not allow direct integration of mass spectrometry ...
Florian Huber, M. Arnaud, Brian J Stevenson, Justine Michaux, Fabrizio Benedetti, J. Thevenet, S. Bobisse, Johanna Chiffelle, Talita Gehert, Markus Müller, HuiSong Pak, Anne I Krämer, Emma Ricart Altimiras, Julien Racle, Marie Taillandier-Coindard, K. Muehlethaler, Aymeric Auger, Damien Saugy, Baptiste Murgues, Abdelkader Benyagoub, D. Gfeller, D. D. Laniti, L. Kandalaft, Blanca Navarro Rodrigo, H. Bouchaab, S. Tissot, G. Coukos, A. Harari, M. Bassani-Sternberg   +28 more
semanticscholar   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Using fast and accurate simulation to explore hardware/software trade-offs in the multi-core era [PDF]

, 2011
Writing well-performing parallel programs is challenging in the multi-core processor era. In addition to achieving good per-thread performance, which in itself is a balancing act between instruction-level parallelism, pipeline effects and good memory ...
Carlson, Trevor, Eeckhout, Lieven, Ghysels, Pieter, Heirman, Wim, Sarkar, Souradip, Vanroose, Wim   +5 more
core   +1 more source

DFAST: a flexible prokaryotic genome annotation pipeline for faster genome publication

Bioinform., 2017
Summary: We developed a prokaryotic genome annotation pipeline, DFAST, that also supports genome submission to public sequence databases. DFAST was originally started as an on‐line annotation server, and to date, over 7000 jobs have been processed since ...
Yasuhiro Tanizawa, Takatomo Fujisawa, Yasukazu Nakamura   +2 more
semanticscholar   +1 more source

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source