Results 31 to 40 of about 7,494 (229)

An end-member based ordering relation for the morphological description of hyperspectral images

open access: yes, 2014
Despite the popularity of mathematical morphology with remote sensing image analysis, its application to hyperspectral data remains problematic. The issue stems from the need to impose a complete lattice structure on the multi-dimensional pixel value ...
N. Courty   +5 more
core   +3 more sources

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

open access: yesFEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj   +3 more
wiley   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Dynamic Hybrid Reversible Data Hiding Based on Pixel-value-ordering

open access: yes電腦學刊, 2023
<p>Reversible data hiding (RDH) using pixel-value-ordering (PVO) is a well-established technique for embedding data in a cover image by modifying the maximum and minimum in each block. This paper proposes a dynamic hybrid RDH method based on PVO.
Fang Ren Fang Ren   +2 more
openaire   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Applicability of mitotic figure counting by deep learning: a development and pan‐cancer validation study

open access: yesFEBS Open Bio, EarlyView.
In this study, we developed a deep learning method for mitotic figure counting in H&E‐stained whole‐slide images and evaluated its prognostic impact in 13 external validation cohorts from seven different cancer types. Patients with more mitotic figures per mm2 had significantly worse patient outcome in all the studied cancer types except colorectal ...
Joakim Kalsnes   +32 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

A generalized pixel value ordering data hiding with adaptive embedding capability

open access: yes, 2022
In this paper, we proposed an improvisation to Li et al.’s pixel value ordering (PVO) method and called it as generalized PVO (GePVO), which provides a suitable expansion strategy using the image redundancy more abundantly. GePVO makes use of a reference
Yu-Ju Tseng   +4 more
core  

T1 Over Squared Proton Density Ratio to Characterize Multiple Sclerosis Lesions

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Differentiating remyelinated from demyelinated lesions in MS remains challenging without histological confirmation. This study introduces the T1‐to‐PD2 ratio (TPR) imaging approach and evaluates its ability to characterize MS lesions alongside other quantitative MRI (qMRI) metrics. Methods Thirty individuals with MS (mean age: 47.5 ± 
Sarah J. Wright   +10 more
wiley   +1 more source

Overlapping pixel value ordering predictor for high-capacity reversible data hiding

open access: yes, 2023
In recent years, many reversible information hiding methods have been proposed. Among them, the pixel value ordering (PVO) method can be used to create high-fidelity camouflage images under good embedding capacity.
Chin-Feng Lee   +3 more
core  

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