Results 61 to 70 of about 282,600 (283)

Modelling stem cell differentiation related processes—A practical overview for biologists

FEBS Letters, EarlyView.
Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar, Xinqi Yan, Lucas Jansen Klomp, Stefano Schivo, Janine N. Post   +4 more
wiley   +1 more source

Plant Biotechnology: Applications in In Vitro Plant Conservation and Micropropagation

Horticulturae
Micropropagation and conservation using in vitro methods are one of the most important technologies used to maintain the gene pool and genetic diversity [...]
Rupesh Kumar Singh, Vasiliy A. Chokheli
doaj   +1 more source

Mixed‐class J‐domain protein scaffolds promote expanded aggregate handling and multivalent Hsp70 engagement during functional disaggregase assembly

FEBS Letters, EarlyView.
Protein aggregates threaten proteostasis and cell health. In human cells, Hsp70–J‐domain protein‐based disaggregases remove aggregates, but how they assemble remains unclear. Our biochemical findings show that DNAJA2‐ and DNAJB1‐containing disaggregase scaffolds enhance luciferase aggregate targeting, and that Hsp70 recruitment by both J‐domain ...
Anna Szlachcic, Nadinath B. Nillegoda
wiley   +1 more source

Aptamers as emerging tools for plant disease management: From biosensing to active modulation

iScience
Summary: Conventional methods for plant disease detection and management often face limitations in sensitivity, specificity, and field applicability. Aptamers, with high affinity and specificity, present a promising alternative.
Fuguo Liu, Yu Zhang, Zhixuan Wei, Hongsen Wu, Yanhui Jia, Xiaofang Li   +5 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Ectopic expression of specific GA2 oxidase mutants promotes yield and stress tolerance in rice

, 2017
A major challenge of modern agricultural biotechnology is the optimization of plant architecture for enhanced productivity, stress tolerance and water use efficiency (WUE).
Hsieh, Kun-Ting, Chen, Chi-Yu, Jiang, Mirng-Jier, Huang, Tzu-Pi, Sakakibara, Hitoshi, Tuan‐Hua David Ho, Lin‐Chih Yu, Miin‐Huey Lee, Ku‐Ting Chen, Yu, Su-May, Shuen‐Fang Lo, Mirng‐Jier Jiang, Lo, Shuen-Fang, Su‐May Yu, Lee, Miin-Huey, Ho, Tuan-Hua David, 陳良築, Chi‐yu Chen, Chen, Ku-Ting, Mikiko Kojima, Kun‐Ting Hsieh, Tzu‐Pi Huang, Liu, Yi-Lun, Kojima, Mikiko, Hitoshi Sakakibara, Yi‐Lun Liu, Yu, Lin-Chih, Liang‐Jwu Chen, Chen, Liang-Jwu   +28 more
core   +1 more source

Evaluation of the knowledge of a population about the risk of plantain and banana transformed with Black Sigatoka resistant genes

Biotecnología Vegetal, 2007
An increasing impact of plant biotechnology on society is present in all over the world. Scientific understanding should be developed on people so they can be able to make right personal decisions and participate in public debate about this technology ...
José M. Machado-Rodríguez
doaj  

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Plant Defense Proteins: Recent Discoveries and Applications

Plants
Proteins play pivotal roles in safeguarding plants against numerous biotic and abiotic stresses. Understanding their biological functions and mechanisms of action is essential for advancing plant biology, agriculture, and biotechnology.
Samuel O. Shobade, Marit Nilsen-Hamilton, Olga A. Zabotina   +2 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source