Plant cell packs: a scalable platform for recombinant protein production and metabolic engineering [PDF]
Industrial plant biotechnology applications include the production of sustainable fuels, complex metabolites and recombinant proteins, but process development can be impaired by a lack of reliable and scalable screening methods. Here, we describe a rapid
Arfi +28 more
core +2 more sources
Optimization of total RNA isolation method from the aromatic medicinal plant Artemisia annua L.
This paper is the first report on the development of a protocol that allows rapid and simplified extraction of total RNA from Artemisia annua L., an aromatic medicinal plant.
Suganthi Appalasamy +5 more
doaj +1 more source
Epigenomic consequences of immortalized plant cell suspension culture. [PDF]
Plant cells grown in culture exhibit genetic and epigenetic instability. Using a combination of chromatin immunoprecipitation and DNA methylation profiling on tiling microarrays, we have mapped the location and abundance of histone and DNA modifications ...
Milos Tanurdzic +8 more
doaj +1 more source
Nutritional phosphate deficiency is a major limitation to plant growth. Here, we monitored fluxes in pathways supporting respiratory metabolism in potato (Solanum tuberosum) cell cultures growing in control or limiting phosphate conditions.
Jiang Zhou He +3 more
doaj +1 more source
Novel applications of plant tissue culture and conventional breeding techniques to space biology research [PDF]
In the minds of many, plant cell and tissue culture may now be viewed as a well-developed technology. It has been all but reduced to a tool to solve practical problems and contribute to plant improvement.
Krikorian, Abraham D.
core +1 more source
Embryogenic cell suspension cultures of Santalum album were transformed with Agrobacterium tumefaciens harboring pD35SHER plant expression vector having hepatitis B small surface antigen (HBsAg) with a C-terminal ER retention signal.
U. K. S. Shekhawat +2 more
doaj +1 more source
The sensitivity of plant tissue culture and plant cell of citrullus lanatus cv. round dragon against basta® [PDF]
Current study provides an efficient screening system for transformed plant of Citrullus lanatus cv. Round Dragon harboring bar gene. The untransformed 5-day-old cotyledon explants were cultured on the shoot-inducing media supplemented with Basta® (0.2, 0.
Huyop, Fahrul Zaman, K., Ganasan
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The untapped potential of plant thin cell layers [PDF]
Thin cell layers (TCLs), which contain a small number of cells or tissues, are explants excised from different organs (stems, leaves, roots, inflorescences, flowers, cotyledons, hypocotyls/epicotyls, and embryos).
Botero Giraldo +15 more
core +1 more source
Nuclear DNA and protein content evaluation in Taxus plant cell cultures using multiparameter flow cytometry [PDF]
Plant cell cultures of Taxus provide the most reliable production methods for the anti-cancer drug paclitaxel. In order to comprehend the inherent culture heterogeneity and production variability in cell cultures, it is essential that the cellular ...
Amit Shavit +2 more
core +2 more sources
Alternative carbon sources for the production of plant cellular agriculture: a case study on acetate
Plant cellular agriculture aims to disrupt the way plant derived products are produced. Plant cell cultures are typically grown with sucrose as the primary carbon and energy source, but alternative carbon sources may have advantages over sucrose ...
Elizabeth C. Hann +9 more
doaj +1 more source

